Sequences from confirmation amplicon


Confirmation sequences for line SALK_148550c and hit At5g10270 are shown.
Line ID SALK_148550c
Locus At5g10270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3224877
Primers used for PCR 28hg - LBb3, show primer details
   
Sequence Name 28-K112874-SALK_148550C-28hg
GenBank Accession LN485087 [GenBank]
Sequencing primer gene specific primer, 28hg: 5'-GTAGATGCCACCCTTGTATTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K112874-SALK_148550C-28hg
CTTAGTTAGAAGAATCCTCAATAAGCAGAACTAAGACAGTAGTTGAAATCACTTTTTTTC
ACAAACCTGGCTTTCCTTGGTCATCCCTGTCCCGACCTTTTAAGAGACAGAACCATTCAA
AACACAAAATGTTTAGACCTCTTTATATAGAAATAGCTAACTTAATTCAGTGCAACATAT
GTGTTCCTTAAACAAACCTGGTGAAGTCACAATCTCTTTCAGCTGAATGACATTTTCATG
ATGAAGCTTCTTCAGAATCTTTATCTCTCTAATAGCTGTTATAGGAAACTGCATCATACC
AGTTGTAAACAATGGGTTACAATTCCTATCGTAAACAAAAACTTGCATCTGTTCTAAACA
TAGTGAGCATCATAAAAAAAACGTATAGTCCATATAAAGTGAAGAATTCACATACCCCTT
CTCTTTCATTGTCCATACGTATCTTTTTGAGAGCCACAATTTCACCAGTTTTGATTTCTT
TAGCCATGTAAACTTGACTGCAACAGCAAAACAACAACAATCAAATCTCCTAAAAGCAAC
ACAACATAGGCATTGTAAAAGCCTAATCATACAATCAAAAATCGCTTCAAAGGCCAATAA
TTTATCAATCCCAAGTAAAAATTAAGCCTTTTGAATAACCAAAGAGTGAAATTGAATTAA
AGAGAAAGTGAATAACCCGTAAGTGCCTTCACCAATTTGTTCGAGCTTCTCAAAGCAATC
AACGCTGCGAGATCCCCAGATTGGAGGAGGTTCCTCGAGATTTAGCTGCCCGAATGATGC
CATCGCCATCTTCGTTCCCGGGAGTCCCACTGTCGAACAATATCTTTTACTCCGATGATC
AAATTACGTCTTTCTCGATCGCTTTCCTTTTCTCTCAGACAACTCTCTCTCCGTAGGTTT
CCCAGCTTATTTATGTAATTAGCGTTTCGGTTTAAATCTATGAATTGTCCGGTTTGAAAC
GGTCTAACCCTGAACAAGCGGTTTGGTGTAAATTAGCCGCTTAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
   
Sequence Name 20-K112873-SALK_148550C-R210
GenBank Accession LN484851 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K112873-SALK_148550C-R210
TATTTCACCACCGCTTGTTCGGGTTAGACGCGGTTCAACCGGACAATTCATAGCCCTTTA
CCGAAACGCTAATTACATAAATAAGCTGGGAAACCTACGGAGAGAGAGTTGTCTGAGAGA
AAAGGAAAGCGATCGAGAAAGACGTAATTTGATCATCGGAGTAAAAGATATTGTTCGACA
GTGGGACTCCCGGGAACGAAGATGGCGATGGCATCATTCGGGCAGCTAAATCTCGAGGAA
CCTCCTCCAATCTGGGGATCTCGCAGCGTTGATTGCTTTGAGAAGCTCGAACAAATTGGT
GAAGGCACTTACGGGTTATTCACTTTCTCTTTAATTCAATTTCACTCTTTGGTTATTCAA
AAGGCTTAATTTTTACTTGGGATTGATAAATTATTGGCCTTTGAAGCGATTTTTGATTGT
ATGATTAGGCTTTTACAATGCCTATGTTGTGTTGCTTTTAGGAGATTTGATTGTTGTTGT
TTTGCTGTTGCAGTCAAGTTTACATGGCTAAAGAAATCAAAACTGGTGAAATTGTGGCTC
TCAAAAAGATACGTATGGACAATGAAAGAGAAGGGGTATGTGAATTCTTCACTTTATATG
GACTATACGTTTTTTTTATGATGCTCACTATGTTTAGAACAGATGCAAGTTTTTGTTTAC
GATAGGAATTGTAACCCATTGTTTACAACTGGTATGATGCAGTTTCCTATAACAGCTATT
AGAGAGATAAAGATTCTGAAGAAGCTTCATCATGAAAATGTCATTCAGCTGAAAGAGATT
GTGACTTCACCAGGTTTGTTTAAGGAACACATATGTTGCACTGAATTAAGTTAGCTATTT
CTATATAAAGAGGTCTAAACATTTTGTGTTTTGAATGGTTCTGTCTCTTAAAAGGTCGGG
ACAGGGATGACCAAGGAAGGCCAGGTTTGTGAAAAAAAGTGGATTTCACTACTGTCTTAG
TTTCCTGCTAATGGAGGATTTCTTCCTTACTA

Last Updated on 06.12.2018 16:11