Sequences from confirmation amplicon


Confirmation sequences for line SALK_148625c and hit At3g59140 are shown.
Line ID SALK_148625c
Locus At3g59140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21864462
Primers used for PCR 49um - LBb3, show primer details
   
Sequence Name 92-K117830-SALK_148625C-49um
GenBank Accession LN486348 [GenBank]
Sequencing primer gene specific primer, 49um: 5'-GGTATGGCGCTATCTTATGGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K117830-SALK_148625C-49um
TTACTTAGCTAACTGGATCATTTCGGTTGAGAGACTTAATCAGTACACACATTTAACACC
TGAGGCTCCTGAAGTAATAGAAGAGACTCGACCACCGGTTAATTGGCCGGTCACAGGTCG
AGTCGAAATCTCGGATTTGCAGGTAAGTTTGATTAATACACTTTCACTCTCTAATCCATA
TTATGAGTGTTTCTTAATTTATTAATGTTCATATATACAGATAAGATACAGAAGAGAATC
CCCACTGGTTCTAACACATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAAT
   
Sequence Name 75-K117829-SALK_148625C-R210
GenBank Accession LN486138 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K117829-SALK_148625C-R210
AACCAGTGGGGATTCTCTTCTGTATCTTATCTGTATATATGAACATTAATAAATTAAGAA
ACACTCATAATATGGATTAGAGAGTGAAAGTGTATTAATCAAACTTACCTGCAAATCCGA
GATTTCGACTCGACCTGTGACCGGCCAATTAACCGGTGGTCGAGTCTCTTCTATTACTTC
AGGAGCCTCAGGTGTTAAATGTGTGTACTGATTAAGTCTCTCAACCGAAATGATCCAGTT
AGCTAAGTAACATTGGTTCTGAACAGAGTAAACAAGTCCCATATTCAAAGATAAACCATA
AGATAGCGCCATACC

Last Updated on 06.12.2018 16:11