Sequences from confirmation amplicon


Confirmation sequences for line SALK_148834c and hit At5g52540 are shown.
Line ID SALK_148834c
Locus At5g52540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21321669
Primers used for PCR SC10 - R204, show primer details
   
Sequence Name 01-K107118-SALK_148834C-R210
GenBank Accession LN484267 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K107118-SALK_148834C-R210
TCTTCACATGAACTTAAGAACTTTAACTCCAAATGCAATTCCTATGAAAGTTGCAATGGC
GATCCCAAATATTCCGGCCAGAATCCCGGGAACTATCAACGAGTTCCATCCCTTTGCCGT
CGCCATTCCAGCTGCGGTCGTGGGTCCTCCGACATTAGCATTTGATGCTAGAAGCAGTAA
CCTTAGCTCGATGTTTAGCAGCTTTCCTATGCCCAATATCACAGCCAGATGTGTCCCAAT
TTGAACCAATGCAAACAAGAATATGCTCGGCGCCGTGTTGATCACACTCCATATGTTTCC
GCTTGCACCCACCACTGTGAAGAACACCTGAAAGAAAACTAGCTTGATTAATATCAAGAA
GGTTGCATCGTTCTTGAATTTCTTGTCTCATACCTGCATCAAAATTAGAGCCATGGCTTC
TCCAGATGGAGCAAGACGACCAAACTGGGACGGAAAGACAGTTGCTAAAATAACAACAAC
CGCTGTTATAGCCGGTAAGCTACCACCCGAAATTCCGAAGTACTTTGTTAGCAAAGCCCC
AGCCTTGCAGATAGCTAATGACAC
   
Sequence Name 17-K107119-SALK_148834C-SC10
GenBank Accession LN484734 [GenBank]
Sequencing primer gene specific primer, SC10: 5'-GCTACTGGAATTGCTGTGTCATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K107119-SALK_148834C-SC10
GAAATCGGGTGGTAGCTTACCGGCTATAACAGCGGTTGTTGTTATTTTAGCAACTGTCTT
TCCGTCCCAGTTTGGTCGTCTTGCTCCATCTGGAGAAGCCATGGCTCTAATTTTGATGCA
GGTATGAGACAAGAAATTCAAGAACGATGCAACCTTCTTGATATTAATCAAGCTAGTTTT
CTTTCAGGTGTTCTTCACAGTGGTGGGTGCAAGCGGAAACATATGGAGTGTGATCAACAC
GGCGCCGAGCATATTCTTGTTTGCATTGGTTCAAATTGGGACACATCTGGCTGTGATATT
GGGCATAGGAAAGCTGCTAAACATCGAGCTAAGGTTACTGCTTCTAGCATCAAATGCTAA
TGTCGGAGGACCCACGACCGCAGCTGGAATGGCGACGGCAAAGGGATGGAACTCGTTGAT
AGTTCCCGGGATTCTGGCCGGAATATTTGGGATCGCCATTGCAACTTTCATAGGAATTGC
ATTTGGAGTTAAAGTTCTTAAGTTCATGTGAAGATGGTAGTATAACACATTGAGACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG

Last Updated on 06.12.2018 16:11