Sequences from confirmation amplicon


Confirmation sequences for line SALK_149104 and hit At1g08940 are shown.
Line ID SALK_149104
Locus At1g08940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2878785
Primers used for PCR 68us - LBb3, show primer details
   
Sequence Name 37-K117668-SALK_149104C-R210
GenBank Accession LN485256 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117668-SALK_149104C-R210
ACCTAGTGATTGTGTCCCACGGACTGACATCTCGAGTGTTTCTACAAAATGGTTCAAGTG
GACGGTGGCAGAGTTCGAGCGGTTGAATAACTTTGGGAACTGCGAGTTCAGAGTGATGGA
GTTGGGTGCGAGCGGAGAGTACACTTTCGCGATACACCACAGTGAAGAAGAGATGTTGGA
TTGGGGCATGTCTAAAGATATGATTGATGATCAAAAGGATCGTGTTGATGGGTGTCGTGT
AACAACATCAAACGATTCTTGTTCATTGCATCTCAATGAGTATTTTGATTTGTTAGATGT
CACTGATGATGAAGAATGACCACTAAATTTATATCGCTATACTTTGTTTTGCGTATTGAA
AGTTTATAATATTTATTTCATTTGTTTGTTGGTTTTGTTAAATACCGAGTTTGTTATCCA
AGATCGAATGAAAATTCAC
   
Sequence Name 74-K117669-SALK_149104C-68us
GenBank Accession LN486116 [GenBank]
Sequencing primer gene specific primer, 68us: 5'-GTGAATTTCAATTCGATCTTGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K117669-SALK_149104C-68us
ACACAACAATGAAATAATATTATAAACTTTCAATACGCAAAACAAAGTATAGCGATATAA
ATTTAGTGGTCATTCTTCATCATCAGTGACATCTAACAAATCAAAATACTCATTGAGATG
CAATGAACAAGAATCGTTTGATGTTGTTACACGACACCCATCAACACGATCCTTTTGATC
ATCAATCATATCTTTAGACATGCCCCAATCCAACATCTCTTCTTCACTGTGGTGTATCGC
GAAAGTGTACTCTCCGCTCGCACCCAACTCCATCACTCTGAACTCGCAGTTCCCAAAGTT
ATTCAACCGCTCGAACTCTGCCACCGTCCACTTGAACCATTTTGTTAGAAACACTCGAGA
TGTCAGTCCGTGGGACACAATCACTAGGTTTAGTTCACTTGATGGATCCACTTGATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATGGGGCAAAAT

Last Updated on 06.12.2018 16:11