Sequences from confirmation amplicon


Confirmation sequences for line SALK_149589c and hit At3g06060 are shown.
Line ID SALK_149589c
Locus At3g06060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1830095
Primers used for PCR 65qz - LBb3, show primer details
   
Sequence Name 87-K116055-SALK_149589C-65qz
GenBank Accession LN486309 [GenBank]
Sequencing primer gene specific primer, 65qz: 5'-TAAAGATGGGAAATTTGAGAGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K116055-SALK_149589C-65qz
TGGAGCAGGTCCCGATCTTTCCTTGCTTTCATGGCAGGCAAAGCGGCTTTAATCACATTG
AAGCTCCCAACAAGATTTACGTCAATCGTGAATTTGACATCTTCAGGACTATGTTTCACC
AGCTCCTTCGCCGTAAACACACCTTGGTTAACAATCAACACATCGATCGGTCCCGATTCA
TCTATCGCCTTCGAGACTGCATCGTAATCGCGAACGTCTGCAGAGAATGTGGCGACTTCG
ACACCAGTGGCGAGCTGGATCGATTTCTTAGCCTCCTCGAGCTTTGATCCGGATCGGGCG
AGGATTGAGACACGAGCGCCCTCGGAAGCGGCGCGGTGGGCGAGAGCAAGACCGATGCCG
CTAGATCCACCGGTGATGAAGGCGTGACGGGACTTGATAGGGATCTTAATGGGACGAGGA
CGTACAATTAGGGCTAAGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 60-K116054-SALK_149589C-R210
GenBank Accession LN485749 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K116054-SALK_149589C-R210
GTCATTTGTTTACCCACAATATATCTTAGCCCTAATTGTACGTCCTCGTCCCATTAAGAT
CCCTATCAAGTCCCGTCACGCCTTCATCACCGGTGGATCTAGCGGCATCGGTCTTGCTCT
CGCCCACCGCGCCGCTTCCGAGGGCGCTCGTGTCTCAATCCTCGCCCGATCCGGATCAAA
GCTCGAGGAGGCTAAGAAATCGATCCAGCTCGCCACTGGTGTCGAAGTCGCCACATTCTC
TGCAGACGTTCGCGATTACGATGCAGTCTCGAAGGCGATAGATGAATCGGGACCGATCGA
TGTGTTGATTGTTAACCAAGGTGTGTTTACGGCGAAGGAGCTGGTGAAACATAGTCCTGA
AGATGTCAAATTCACGATTGACGTAAATCTTGTTGGGAGCTTCAATGTGATTAAAGCCGC
TTTGCCTGCCATGAAAGCAAGGAAAGATCGTGGACCTGCTTCCATCAGTCTCGTCTCCTC
TCAAGCTGGTCAGGTTCGTCTCTCAAATTTCCCATCTTTAAAA

Last Updated on 06.12.2018 16:11