Sequences from confirmation amplicon


Confirmation sequences for line SALK_149928c and hit At5g47550 are shown.
Line ID SALK_149928c
Locus At5g47550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19286951
Primers used for PCR CG15 - LBb3, show primer details
   
Sequence Name 17-K108444-SALK_149928C-CG15
GenBank Accession LN484738 [GenBank]
Sequencing primer gene specific primer, CG15: 5'-CATGTAAATGAGCAATATGGATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K108444-SALK_149928C-CG15
CTTCCTTTCTTTCTTTCCCTCACATGTTCCCTCTTGTCTCCTGTTTTCGTTTTGACTCAT
TTAATTGTTTTATTTAGAGAGTGTTATTACATCTCTCAACTGTCTCGTGACTGACCCATA
ATTTGCATACCAGATTATCCGGCATAATTACCGGATTTGTCAAAGAACTTGTTCAATCAT
CATTTCTTCAGATTTGCATATTTTGAAAACTATAGAAATACATAAAATAGACACAATAAT
TGTCTTATGTTTATTTCAACTCCCTTGTTTTTAATGTTTCTGCAATACTACTAAAGAGCG
GTTCCTTGCTAAACCCTTATA
   
Sequence Name 01-K108443-SALK_149928C-R210
GenBank Accession LN484269 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K108443-SALK_149928C-R210
GTCATTTGTTACCCACAGTATAACTCAATGCCATAATATCCTCTCTCTCGCCCCTAAAAC
CTCCCTCTCTACGCCTCTGCGGCTGCCCGCGTCCGTGGTTGGAGTCCCATCAGCAACGTT
ACGGACCCTCAAGTCGTAGAGATCGGTGAATTCGCGGTCTCCGAGTACAACAAACGGAGC
GAATCGGGACTCAAGTTCGAGACGGTTGTTAGCGGCGAGACTCAGGTCGTATCTGGTACA
AATTACCGTCTTAAGGTGGCGGCTAATGACGGCGACGGAGTGAGCAAGAACTACTTGGCA
ATCGTATGGGACAAGCCGTGGATGAAATTCAGAAATCTCACTTCCTTCGAGCCTGCCAAT
AATGGTCGCTTCCTTTAAGCTTTTAACATGTAACCGCTCTTTATTAATATTGCAGAAACA
TTAAAAACAAAGGAGTTGAAATAAACATAAGACAATTATTGTGTCTATTTTATGTATTCC
TATAGTTTTCACAATATGCCAATCT
Primers used for PCR KC16 - R212, show primer details
   
Sequence Name 84-K119135-SALK_149928C-KC16
GenBank Accession LN486279 [GenBank]
Sequencing primer gene specific primer, KC16: 5'-CATCAATGCATGGTTTATAACGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K119135-SALK_149928C-KC16
CTTTGTCCTTTGCTATACACTACTTGTCTATTGAATAATATGCTTATAATCGTAAAAAAC
TACAAGTAGTCATAATCCTTTTGGATATTTATTTTCGTAGGTAGAAGTTGGCCATTGCAA
ATTACATAAATGATATCCAACCTCTAGAGGATAAACCTCATTAATTTGTCTATATTATGC
TGAATTCCATAACATATAAAGAATTATGTGAGTGTTAATTATTTACCAATAAATTAGTAT
AAATTTTGAAATACAAAGCAAAAAAGTTTTTCATAAAAGTTCATTAAACCTTTTGCCTAA
ATTATGGCATGTGTTATTAAGATTAGAAATAAATATTCTGCTAGAAATCTAGAATTCTGC
CACACGTCATGCCACGTGGCGGACCTTTAATGTATTTAAAAGCCCTTAAACTCAATGCCA
TAATCAATTTTGAGGAAACAACAGTTACAAAGCAAAATGACTAGTAAGGTGAAGGCGGGA
AACGACAATCTGATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGA
CGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTC
AGCCGCGGGTTTCTGGAGTTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGCGCG
TTCAAAAGT
   
Sequence Name 70-K119134-SALK_149928C-R214
GenBank Accession LN486018 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K119134-SALK_149928C-R214
GGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGCCT
TCACCTTACTAGTCATTTTGCTTTGTAACTGTTGTTTCCTCAAAATTGATTATGGCATTG
AGTTTAAGGGCTTTTAAATACATTAAAGGTCCGCCACGTGGCATGACGTGTGGCAGAATT
CTAGATTTCTAGCAGAATATTTATTTCTAATCTTAATAACACATGCCATAATTTAGGCAA
AAGGTTTAATGAACTTTTATGAAAAACTTTTTTGCTTTGTATTTCAAAATTTATACTAAT
TTATTGGTAAATAATTAACACTCACATAATTCTTTATATGTTATGGAATTCAGCATAATA
TAGACAAATTAATGAGGTTTATCCTCTAGAGGTTGGATATCATTTATGTAATTTGCAATG
GCCAACTTCTACCTACGAAAATAAATATCCAAAAGGATTATGACTACTTGTAGTTTTTTA
CGATTATAAGCATATTATTCAATAGACAAGTAGTGTATAGCAAAGGACAAAGAAAGAACA
TAAATGAGTGTAAAGGACAAAAACTAATTAAAAAGCAAGACGTTATAAACCAGNCATTGA
T

Last Updated on 06.12.2018 16:11