Sequences from confirmation amplicon


Confirmation sequences for line SALK_150134c and hit At3g60630 are shown.
Line ID SALK_150134c
Locus At3g60630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22410854
Primers used for PCR 25lu - LBb3, show primer details
   
Sequence Name 40-K115696-SALK_150134C-25lu
GenBank Accession LN485295 [GenBank]
Sequencing primer gene specific primer, 25lu: 5'-CTTAACAACAACAACGACGACACT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K115696-SALK_150134C-25lu
ATATAACAGAAGCTCTTCACTCTCTCCTTCAAGACTCATCATTATCACCACCGGTCTCTC
TCACCTCCTCAAAACCTAATCTTTCGGATCGCAGCTTACAGAGCTTTCTCAGAAACGTCA
CCGTTTCTTCAATTCGTCAACTTCACAGCAAACCAAACGATTCTCGAGTCATTCGAAGGG
TTTGATCGGATCCACATTGTCGATTTCGATATCGGTTATGGAGGTCAATGGGCGTCTCTG
ATTCAAGAGCTCGCCGGAAAAAGAAACAGATCTTCATCAGCTCCGTCGCTAAAGATTACA
GCTTTCGCTTCTCCTTCAACTGTCTCCGACGAATTCGAGCTCCGATTCACTGAAGAAAAT
CTCAGAAGCTTCGCCGGCGAAACAGGTGTCTCCTTCGAAATCGAGCTCTTAAACATGGAG
ATTCTCTTGAATCCAACTTATTGGCCACTGTCTTTATTCCGATCATCGGAGAAAGAAGCA
ATCGCTGTGAATCTCCCAATCAGCTCCATGGTCTCCGGTTACCTCCCATTGATACTTCGT
TTCCTCAAGCAAATCTCACCAAACGTCGTCGTTTGCTCAGACAGAAGCTGCGACCGTAAC
AACGACGCGCCGTTCCCTAACGGTGTAAACAAATTGACGCTNAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAG
CTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTNTG
CCCCAGCAGGCGAANATCCTGTTTGATGGTGGTTCC
   
Sequence Name 29-K115695-SALK_150134C-R210
GenBank Accession LN485112 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K115695-SALK_150134C-R210
TTTGTTTCCCGTTAGGGAACGGCGCGTCGTTGTTACGGTCGCAGCTTCTGTCTGAGCAAA
CGACGACGTTTGGTGAGATTTGCTTGAGGAAACGAAGTATCAATGGGAGGTAACCGGAGA
CCATGGAGCTGATTGGGAGATTCACAGCGATTGCTTCTTTCTCCGATGATCGGAATAAAG
ACAGTGGCCAATAAGTTGGATTCAAGAGAATCTCCATGTTTAAGAGCTCGATTTCGAAGG
AGACACCTGTTTCGCCGGCGAAGCTTCTGAGATTTTCTTCAGTGAATCGGAGCTCGAATT
CGTCGGAGACAGTTGAAGGAGAAGCGAAAGCTGTAATCTTTAGCGACGGAGCTGATGAAG
ATCTGTTTCTTTTTCCGGCGAGCTCTTGAATCAGAGACGCCCATTGACCTCCATAACCGA
TATCGAAATCGACAATGTGGATCCGATCAAACCCTTCGAATGACTCGAGAATCGTTTGGT
TTGCTGTGAAGTTGACGAATTGAAGAAACGGTGACGTTTCTGAGAAAGCTCTGTAAGCTG
CGATCCGAAAGATTAGGTTTTGAGGAGGTGAGAGAGACGGTGGTGATAATGATGAGTCTT
GAAGGAGAGAGTGAAGAGCTTCTGTTATATACGAAGCTGCTCTGTGGAACGGAGGTTTAG
GATTGTTGTTAGTGTCGTCGTTGTTGTTGTTAAG

Last Updated on 06.12.2018 16:11