Sequences from confirmation amplicon


Confirmation sequences for line SALK_150343c and hit At3g10630 are shown.
Line ID SALK_150343c
Locus At3g10630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3321811
Primers used for PCR 06gj - LBb3, show primer details
   
Sequence Name 30-K112129-SALK_150343C-06gj
GenBank Accession LN485128 [GenBank]
Sequencing primer gene specific primer, 06gj: 5'-TGTTGATGTTGATATAGGGTGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K112129-SALK_150343C-06gj
CTGAGAGAGCTTAAGAAGAGACTGGAGTTCCTCTGCTGAGATTCTCGCTGAGGTGTCTTT
AGCTTCTTCTTCTTCTTCGTCTTCTTAAACATTCAACAATTTAAGAATTCGTCTAAAGAA
CTCTTTTTTGTTGTTGTTGTTACTGTAGGCACCTCACTGGATAACCTATGACTTCCCTCC
TGATGTTAGGGAGAAGCTTAAGGTCCGATGGGGATCCGATTGGAAGGGTCAAGCACAGAA
ATGGTGAGTCTTTCTGAGATTATCAAGAATCATGTCATTTCATTACGGGCTTGTTCAAAT
AATGTGTTTCCTCAGGTTTCTTCTGAAATTTACTGGGAAAGATGAAGAAATCAATCTTCT
TGGCGATGGTACTGAGAAACCTGAGTTTGGTGAATGGTCCTGGACTTCTCCTGATCAAGT
CGTTGAAAATGTACGTACAGTTTTCTTCTCTCTGTTTGACCTTCAGCATTTAGATTCCAC
GACTACTCTNTCACTCATATTCATTCTATTTCTGTTATACAGGCAGTGGAGTTCAAGAAG
CCAGTGTACAAGGAAGTCATGTCAGCTTTCGCTTCTCATCTCCAGTAGCAAAATAAATCG
AAAACTAAACTCTCTGTTTTAAGTTTTTCGTAAGAAACTCTGAGAATAAGCTCTTCTTGA
CATATTTGCTCTTTTTTGAACAATCTTTGAATCAAAATACAATTCTTGTTCCCTCTCTNG
ATAAAAGTAAAGTGTGAGATCCTGTCATGTTCTTATTTTCTCATCANAGATTCTCGCTAT
TTGATCCGCTACAACCTTAGCAACAAATTGACACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGNGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCNGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTNTGCCCCAGCAGGCGAAN
ATCCTGTTTGATGG
   
Sequence Name 14-K112128-SALK_150343C-R210
GenBank Accession LN484667 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K112128-SALK_150343C-R210
AGCGGATCAATAGCGAGAATCTTTGATGAGAAAATAAGAACATGACAGGATCTCACACTT
TACTTTTATCAAGAGAGGGAACAAGAATTGTATTTTGATTCAAAGATTGTTCAAAAAAGA
GCAAATATGTCAAGAAGAGCTTATTCTCAGAGTTTCTTACGAAAAACTTAAAACAGAGAG
TTTAGTTTTCGATTTATTTTGCTACTGGAGATGAGAAGCGAAAGCTGACATGACTTCCTT
GTACACTGGCTTCTTGAACTCCACTGCCTGTATAACAGAAATAGAATGAATATGAGTGAA
AGAGTAGTCGTGGAATCTAAATGCTGAAGGTCAAACAGAGAGAAGAAAACTGTACGTACA
TTTTCAACGACTTGATCAGGAGAAGTCCAGGACCATTCACCAAACTCAGGTTTCTCAGTA
CCATCGCCAAGAAGATTGATTTCTTCATCTTTCCCAGTAAATTTCAGAAGAAACCTGAGG
AAACACATTATTTGAACAAGCCCGTAATGAAATGACATGATTCTTGATAATCTCAGAAAG
ACTCACCATTTCTGTGCTTGACCCTTCCAATCGGATCCCCATCGGACCTTAAGCTTCTCC
CTAACATCAGGAGGGAAGTCATAGGTTATCCAGTGAGGTGCCTACNGTAACAACAACAAC
AAAAAAGAGTTCTTTAGACGAATTCTTAAATTGTTGAATGTTTAAGAAGACGAAGAAGAA
GAAGAAGCTNAAGACACCTCAGCGAGAATCTCAGCAGAGTGAACTCCAGTCTCTTCTTTA
AGCTCTCTCATGACTGCAACTCTGGGATCTTCACCTTCGTCGA
Primers used for PCR PP04 - LBb3, show primer details
   
Sequence Name 61-K118240-SALK_150343C-PP04
GenBank Accession LN485778 [GenBank]
Sequencing primer gene specific primer, PP04: 5'-AAGGTTGGGATGTATTGTTGAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K118240-SALK_150343C-PP04
TCGGCAAAATATTGACGAACGCGATATCCTCGGACAGCGTATTTCGGGAACAAGATTCTT
GATTTCGTAGAAGAAATGAATATAGAAGAGCCTCGGAATGGTTACCCTTTTGTGTATGTG
ATTGATAAACACATTGCTCAAGTTGATTTACCTCGGTTGTACAAAGCGGCTGATGCGTTT
GTGTTGCCTACAAGAGGAGAAGGATGGGGACGACCTATAGTGGAAGCCATGGCTATGTCT
TTGCCTGTGATCACAACAAACTGGTCAGGACCAACAGAGTATCTTACAGAGCGGAATGGT
TACCCGTTGGTGGTAGAGGAGATGAGTGAAGTGAAGGAAGGTCCTTTTGAAGGGCATCAA
TGGGCAGAGCCATCTGTGGATAAGCTGAGGGTTCTAATGAGGCGTGTAATGAGTAACCCT
GATGAGGCTAAGGTTAAAGGAAAACGTGGAAGAGATGACATGGTTAATAACACATTGACG
CTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
ATCGGCAAAAT
   
Sequence Name 38-K118239-SALK_150343C-R210
GenBank Accession LN485273 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K118239-SALK_150343C-R210
CATCTCTTCCACGTTTTCCTTTAACCTTAGCCTCATCAGGGTTACTCATTACACGCCTCA
TTAGAACCCTCAGCTTATCCACAGATGGCTCTGCCCATTGATGCCCTTCAAAAGGACCTT
CCTTCACTTCACTCATCTCCTCTACCACCAACGGGTAACCATTCCGCTCTGTAAGATACT
CTGTTGGTCCTGACCAGTTTGTTGTGATCACAGGCAAAGACATAGCCATGGCTTCCACTA
TAGGTCGTCCCCATCCTTCTCCTCTTGTAGGCAACACAAACGCATCAGCCGCTTTGTACA
ACCGAGGTAAATCAACTTGAGCAATGTGTTTATCAATCACATACACAAAAGGGTAACCAT
TCCGAGGCTCTTCTATATTCATTTCTTCTACGAAATCAAGAATCTTGTTCCCGAAATCGC
TGTCCGAGTGATACGCGTTCGTCAACAAAAACAGAGCAACATTGTCTTCACCAGAAAACT
CACTCAAGTAAGCTTTCAACAATACATCCCAACCTT
Primers used for PCR 18yk - LBb3, show primer details
   
Sequence Name 79-K121398-SALK_150343C-18yk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 18yk: 5'-GTTGTTTGTCATAGTGAGCCTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K121398-SALK_150343C-18yk
TCTTATCTGTGATTGGAAGAACAATGTTTAAGACTGATAGAGTCAATCCAGAGCATGTGA
AGCGATGTAATCAAATGGATCATGTTTGGGTTCCTACTGATTTTCACGTCTCTTCGTTTG
TACAAAGTGGGGTTGATTCATCTAAGGTTGTCAAGATTGTGCAGCCTGTAGATGTCGGTT
TCTTCGATCCTTCAAAGTATAAGCCTTTGGATCTGATGGCGGTTGGGGATTTGGTTTTAG
GTTCTGGGATGAAGAATGGGTTTGTGTTCTTGAGTGTGTTTAAATGGGAGCAGAGGAAAG
GTTGGGATGTATTGTTGAAAGCTTACTTGAGTGAGTTTTCTGGTGAAGACAATGTTGCTC
TGTTTTTGTTGACGAACGCGTATCACTCGGACAGCGATTTCGGGAACAAGATTCTTGATT
TCGTAGAAGAAATGAATATAGAAGAGCCTCGGAATGGTTACCCTTTTGTGTATGTGATTG
ATAAACACATTGCTCAAGTTGATTTACCTCGGTTGTACAAAGCGGCTGATGCGTTTGTGT
TGCCTACAAGAGGAGAAGGATGGGGACGACCTATAGTGGAAGCCATGGCTATGTCTTTGC
CTGTGATCACAACAAACTGGTCAGGACCAACAGAGTATCTTACAGAGCGGAATGGTTACC
CGTTGGTGGTAGAGGAGATGAGTGAAGTGAAGGAAGGTCCTTTTGAAGGGCATCA
   
Sequence Name 68-K121397w-SALK_150343C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K121397w-SALK_150343C-R210
TTTAACCTTAGCCTCATCAGGGTTACTCATTACACGCCTCATTAGAACCCTCAGCTTATC
CACAGATGGCTCTGCCCATTGATGCCCTTCAAAAGGACCTTCCTTCACTTCACTCATCTC
CTCTACCACCAACGGGTAACCATTCCGCTCTGTAAGATACTCTGTTGGTCCTGACCAGTT
TGTTGTGATCACAGGCAAAGACATAGCCATGGCTTCCACTATAGGTCGTCCCCATCCTTC
TCCTCTTGTAGGCAACACAAACGCATCAGCCGCTTTGTACAACCGAGGTAAATCAACTTG
AGCAATGTGTTTATCAATCACATACACAAAAGGGTAACCATTCCGAGGCTCTTCTATATT
CATTTCTTCTACGAAATCAAGAATCTTGTTCCCGAAATCGCTGTCCGAGTGATACGCGTT
CGTCAACAAAAACAGAGCAACATTGTCTTCACCAGAAAACTCACTCAAGTAAGCTTTCAA
CAATACATCCCAACCTTTCCTCTGCTCCCATTTAAACACACTCAAGAACACAAACCCATT
CTTCATCCCAGAACCTAAAACCAAATCCCCAACCGCCATCAGATCCAAAGGCTTATACTT
TGAAGGATCGAAGAAACCGACATCTACAGGCTGCACAATCTTGACAACCTTAGATGAATC
AACCCCACTTTGTACAAACGAAGAGACGTGAAAATCAGTAGGAACCCAAACATGATCCAT
TTGATTACATCGCTTCACATGCTCTGGATTGACTCTATCAGTCTCAAACATTGTTCTA
Primers used for PCR 69yq - LBb3, show primer details
   
Sequence Name 48-K122661-SALK_150343C-69yq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 69yq: 5'-TAGCTAAGGAGACGAAGGAGGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K122661-SALK_150343C-69yq
GACGATGTTGTTTGTCATAGTGAGCCTGGTGCTTGGTATCCACCATTGTTTGAGACTCTA
CCTTGTCCTCCAACTGGCTACGAAGATTTCTTATCTGTGATTGGTAGAACAATGTTTGAG
ACTGATAGAGTCAATCCAGAGCATGTGAAGCGATGTAATCAAATGGATCATGTTTGGGTT
CCTACTGATTTTCACGTCTCTTCGTTTGTACAAAGTGGGGTTGATTCATCTAAGGTTGTC
AAGATTGTGCAGCCTGTAGATGTCGGTTTCTTCGATCCTTCAAAGTATAAGCCTTTGGAT
CTGATGGCGGTTGGGGATTTGGTTTTAGGTTCTGGGATGAAGAATGGGTTTGTGTTCTTG
AGTGTGTTTAAATGGGAGCAGAGGAAAGGTTGGGATGTATTGTTGAAAGCTTACTTGAGT
GAGTTTTCTGGTGAAGACAATGTTGCTCTGTTTTTGTTGACGAACGCGTATCACTCGGAC
AGCGATTTCGGGAACAAGATTCTTGATTTCGTAGAAGAAATGAATATAGAAGAGCCTCGG
AATGGTTACCCTTTTGTGTATGTGATTGATAAACACATTGCTCAAGTTGATTTACCTCGG
TTGTACAAAGCGGCTGATGCGTTTGTGTTGCCTACAAGAGGAGAAGGATGGGGACGACCT
ATAGTGGAAGCCATGGCTATGTCTTTGCCTGTGATCACAACAAACTGGTCAGGACCAACA
GAGTATCTTACAGAGCGGAATGGTTACCCGTTGGTGGTAGAGGAGATGAGTGAAATGAAG
GAAGGTCCTTTTGAAGGGCATCAATGGGCAGAGCCATCTGTGGA
   
Sequence Name 46-K122660-SALK_150343C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K122660-SALK_150343C-R210
CATCTCTTCACGNTTTTCCTTTAACCTTAGCCTCATCAGGGTTACTCATTACACGCCTCA
TTAGAACCCTCAGCTTATCCACAGATGGCTCTGCCCATTGATGCCCTTCAAAAGGACCTT
CCTTCACTTCACTCATCTCCTCTACCACCAACGGGTAACCATTCCGCTCTGTAAGATACT
CTGTTGGTCCTGACCAGTTTGTTGTGATCACAGGCAAAGACATAGCCATGGCTTCCACTA
TAGGTCGTCCCCATCCTTCTCCTCTTGTAGGCAACACAAACGCATCAGCCGCTTTGTACA
ACCGAGGTAAATCAACTTGAGCAATGTGTTTATCAATCACATACACAAAAGGGTAACCAT
TCCGAGGCTCTTCTATATTCATTTCTTCTACGAAATCAAGAATCTTGTTCCCGAAATCGC
TGTCCGAGTGATACGCGTTCGTCAACAAAAACAGAGCAACATTGTCTTCACCAGAAAACT
CACTCAAGTAAGCTTTCAACAATACATCCCAACCTTTCCTCTGCTCCCATTTAAACACAC
TCAAGAACACAAACCCATTCTTCATCCCAGAACCTAAAACCAAATCCCCAACCGCCATCA
GATCCAAAGGCTTATACTTTGAAGGATCGAAGAAACCGACATCTACAGGCTGCACAATCT
TGACAACCTTAGATGAATCAACCCCACTTTGTACAAACGAAGAGACGTGAAAATCAGTAG
GAACCCAAACATGATCCATTTGATTACATCGCTTCACATGCTCTGGATTGACTCTATCAG
TCTCAAACATTGTTCTACCAATCACAGATAAAAAATCTTCGTAGCCAGTTGGAGGACAAG
GTAGAGTCTCAAACAATGGTGGATACCAAGCACCAGGCTCACTATGACAAACAACTATCG
TCTCATTGGGTCTACACTGTTCTCTATACATTTCAATCGCTACTTCCTCGT

Last Updated on 06.12.2018 16:11