Sequences from confirmation amplicon


Confirmation sequences for line SALK_150349 and hit At3g07740 are shown.
Line ID SALK_150349
Locus At3g07740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2472489
Primers used for PCR 55gp - LBb3, show primer details
   
Sequence Name 31-K112129-SALK_150349C-55gp
GenBank Accession LN485146 [GenBank]
Sequencing primer gene specific primer, 55gp: 5'-AGCGGGGAGCTAGAGTAAATAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112129-SALK_150349C-55gp
TGTTTTCATCAGTCCTTACCGGAAGTGGAAAGCATGGTGACTGCATGTAAGCAGAATTGA
AGTGTTTAATACATTCTGTCGTTGTCTTACTACCAACATGGTCTGCAACTTCTTTCCAAT
TGCCAAACCCGTATGTCGCAATGGCCTAACACAGTCAATTTACTTCCCAATAGTCAGATG
GAATCTCTATATAGACATGGGAGTGTACAATCAGAAACGATTAATGATTACCTCAAGAAG
GAGTATCTCTTCATCGGCATTCCAATCAGAAGTAACAAGCGAAAAAGACAAATTGTCCTG
TCAAACAAAAGGGTAGATTCAATACAAACAAAAATACAACCATAGAACATTTTCTTTGTT
TTAAACACAATCACTAATCTAATTACTTTTCAGCTGACAAAGCGTTGAATTGAATTCAAA
TATACTGCTCGGCGTTGGAAACTAACCATAACACGATATGGGTGACTGTTCTTGTGACGA
TTAAGTTCAACGCCGACAGAAAAGCATTCCACACAAAGATCAAAGTCCATACAACAGTAA
AGGCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 15-K112128-SALK_150349C-R210
GenBank Accession LN484695 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K112128-SALK_150349C-R210
GTCATTTGTTACACCACAATATATCCTGGCCTTTACTGTTGTATGGACTTTGATCTTTGT
GTGGAATGCTTTTCTGTCGGCGTTGAACTTAATCGTCACAAGAACAGTCACCCATATCGT
GTTATGGTTAGTTTCCAACGCCGAGCAGTATATTTGAATTCAATTCAACGCTTTGTCAGC
TGAAAAGTAATTAGATTAGTGATTGTGTTTAAAACAAAGAAAATGTTCTATGGTTGTATT
TTTGTTTGTATTGAATCTACCCTTTTGTTTGACAGGACAATTTGTCTTTTTCGCTTGTTA
CTTCTGATTGGAATGCCGATGAAGAGATACTCCTTCTTGAGGTAATCATTAATCGTTTCT
GATTGTACACTCCCATGTCTATATAGAGATTCCATCTGACTATTGGGAAGTAAATTGACT
GTGTTAGGCCATTGCGACATACGGGTTTGGCAATTGGAAAGAAGTTGCAGACCATGTTGG
TAGTAAGACAACGACAGAATGTATTAAACACTTCAATTCTGCTTACATGCAGTCACCATG
CTTTCCACTTCCGGTAAGGACTGATGAAAACACATGATCGTCAGATAGTTGAACCTAGCT
GGTTTATTTACTCTGCTTCCCCGC

Last Updated on 06.12.2018 16:11