Sequences from confirmation amplicon


Confirmation sequences for line SALK_150833c and hit At3g14210 are shown.
Line ID SALK_150833c
Locus At3g14210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4730615
Primers used for PCR 34cy - LBb3, show primer details
   
Sequence Name 20-K109819-SALK_150833C-34cy
GenBank Accession LN484844 [GenBank]
Sequencing primer gene specific primer, 34cy: 5'-AAGACGGTGAACTGAAAACCATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K109819-SALK_150833C-34cy
CATTCGTTTACATTGGACCGATTTTTCCGTTATGTTGTTTGGCCAATCATTGAGCAGCTC
GTAACACTCGTTGCCGGTTTTGTATTCTTGTCTCACGATCGGTAAGCATCCCAATGGTGC
TAGCAATTGAACCACGAATTTACTCGCTCCCAAAGAGTACAACAACTGTAAAATAACTTG
TTATAGTTAGTCATTTGAACATAATTAAAGTGCGAAAAAGCTTGTACATTGGTTGTTTGA
CAACGTACTTTGATGTCGTTCTTTAATCGGTTAATAACGTTAGTGACAAAAGCTTGCTGA
GCAGAAGCGTCTGCATTGGGGTTGGCCTTGGTGAAATTCAAATAATCTTCCGTACCAATG
TATATCATAAACAGTGATTTCTCGATGTAGCTGTCGTTCCAATTCGATTTCATTGCGGTG
TAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCGAAAT
   
Sequence Name 04-K109818-SALK_150833C-R210
GenBank Accession LN484363 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K109818-SALK_150833C-R210
GTCATTTGTTACCCGCAATGAAATCGAATTGGAACGACAGCTACATCGAGAAATCACTGT
TTATGATATACATTGGTACGGAAGATTATTTGAATTTCACCAAGGCCAACCCCAATGCAG
ACGCTTCTGCTCAGCAAGCTTTTGTCACTAACGTTATTAACCGATTAAAGAACGACATCA
AAGTACGTTGTCAAACAACCAATGTACAAGCTTTTTCGCACTTTAATTATGTTCAAATGA
CTAACTATAACAAGTTATTTTACAGTTGTTGTACTCTTTGGGAGCGAGTAAATTCGTGGT
TCAATTGCTAGCACCATTGGGATGCTTACCGATCGTGAGACAAGAATACAAAACCGGCAA
CGAGTGTTACGAGCTGCTCAATGATTTGGCCAAACAACATAACGGAAAAATCGGTCCAAT
GTTAAACGAATTTGCTAAAATTAGTACTAGCCCCTATGGTTTTCAGTTCACCGTC

Last Updated on 06.12.2018 16:11