Sequences from confirmation amplicon


Confirmation sequences for line SALK_150899c and hit At4g31920 are shown.
Line ID SALK_150899c
Locus At4g31920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15444356
Primers used for PCR 10bt - LBb3, show primer details
   
Sequence Name 23-K108069-SALK_150899C-10bt
GenBank Accession LN484937 [GenBank]
Sequencing primer gene specific primer, 10bt: 5'-TTCAGATCAACAACAACATCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K108069-SALK_150899C-10bt
ACAAACTCTCCATGGTAGCTCCCAGTAATCTGTTACTTCTCGAGGGTAACCCGCAGTCAT
CATCTTTACCCTCAAACCCGGGTTTTTCTCCTCATTTCGAGATCAGCAAGCGTCTAGAAC
ATTGGTCAAACGCTGCATTGTCAACCAACATTCCACAGAGTGATGTTCATTCAAAACCTG
ACACCTTGGAATGGAATGCGTTCTGCGACTCAGCTAGTCCGCTAGTAAACCCAAACCTGG
ATACAAATCCGGCATCTCTCTGCAGAAACACGGGTTTTGGATCCACAAATGCTGCACAAA
CAGACTTCTTTTATCCATTACAGATGAATCAGCAGCCTGCAAACAACTCAGGTCCAGTGA
CAGAAGCTCAACTGTTTAGAAGTAGCAATCCAAACGAAGGTTTACTCATGGGACAACAGA
AGCTTCAGAGTGGTTTGATGGCTTCTGATGCTGGTTCCTTAGATGATATAGTCAATTCCT
TAATGACACAGGTATTGTATACAATAAAAACCATTTACTTTTTGCACCTTATTCTTTTGG
AGATTTCTGAAGATTTGTTACTTTGACTGTAACAGGAACAGAGCCAATCTGATTTCTCGG
AAGGTGATTGGGATTTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTG
CGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTG
ATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCC
CAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 34-K108070-SALK_150899C-R210
GenBank Accession LN485197 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K108070-SALK_150899C-R210
CATTTGTTTACCCACAAATCCCAATCACCTTCCGAGAAATCAGATTGGCTCTGTTCCTGT
TACAGTCAAAGTAACAAATCTTCAGAAATCTCCAAAAGAATAAGGTGCAAAAAGTAAATG
GTTTTTATTGTATACAATACCTGTGTCATTAAGGAATTGACTATATCATCTAAGGAACCA
GCATCAGAAGCCATCAAACCACTCTGAAGCTTCTGTTGTCCCATGAGTAAACCTTCGTTT
GGATTGCTACTTCTAAACAGTTGAGCTTCTGTCACTGGACCTGAGTTGTTTGCAGGCTGC
TGATTCATCTGTAATGGATAAAAGAAGTCTGTTTGTGCAGCATTTGTGGATCCAAAACCC
GTGTTTCTGCAGAGAGATGCCGGATTTGTATCCAGGTTTGGGTTTACTAGCGGACTAGCT
GAGTCGCAGAACGCATTCCATTCCAAGGTGTCAGGTTTTGAATGAACATCACTCTGTGGA
ATGTTGGTTGACAATGCAGCGTTTGACCAATGTTCTAGACGCTTGCTGATCTCGAAATGA
GGAGAAAAACCCGGGTTTGAGGGTAAAGATGATGACTGCGGGTTACCCTCGAGAAGTAAC
AGATTACTGGGAGCTACCATTGGAGAGTTTTGTTGTGAAGTAAAGCTTGGAAAAGCCCTG
TTGATGTTGTTGTGGATCTGA

Last Updated on 06.12.2018 16:11