Sequences from confirmation amplicon


Confirmation sequences for line SALK_151204 and hit At1g18840 are shown.
Line ID SALK_151204
Locus At1g18840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6502608
Primers used for PCR AW49 - R204, show primer details
   
Sequence Name 68-K106742-SALK_151204C-R210
GenBank Accession LN485945 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K106742-SALK_151204C-R210
ATTTGTTGTCCTGGTAGAAGGCAAGCTGTTTCGACTCTGTGCTGCCTTTTGAGGAATTGT
CAGATTGCAAGCACTTGCTCGTGGGAGACATATCAGACATTCAGACATTGGAGTTGAAGT
TCAGAGGAAATGTCATTTGCATCATCAGCCTTTAGTAAATGCTCTTCAAGCACTTCTCTG
TGATTTCGGTTTCTTGGCTGAATAGCTCTTAACTTTGATTAATGTTTTTGTCTTATCAGG
AGAATAAAGCCAACTCGGTTGTTGATACACATTCTTACTTGGGAATCAATAAGCTAACAG
GAAATGCTTTTGCTCAGAAGGTATACTTGTTTCTACTATTACTATGTTTGAAAAGTTGAG
AAGACCATGGTGGATGATTCTGTTGCCTTATTCTTATCAGCTTCTAGCTTCATCGCCAAA
TGTGCTGCCTTTGTCCCTCGACAATGATTCTTCCAATTCAATCTGGTTAGAGAACTGGCC
ACCATTTTGCTTCTGGAAACCATTTCACTCAGCCCAAGAAAGCATCACTCTCAAAACCT
   
Sequence Name 84-K106743-SALK_151204C-AW49
GenBank Accession LN486266 [GenBank]
Sequencing primer gene specific primer, AW49: 5'-CCTCGACTATCTGAGGATTACTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K106743-SALK_151204C-AW49
AACAAGCGGTCCACACAANCAAGATGCTGACCCCTTATATAACCAGATTGAATTGGAAGA
ATCATTGTCGAGGGCCTTAGGCAGCACGTTTGGCGATGAAGCTAGAAGCTGATAAGAATA
AGGCAACAGAATCATCCACCATGGTCTTCTCAACTTTTCAAACATAGTAATAGTAGAAAC
AAGTATACCTTCTGAGCAAAAGCATTTCCTGTTAGCTTATTGATTCCCAAGTAAGAATGT
GTATCAACAACCGAGTTGGCTTTATTCTCCTGATAAGACAAAAACATTAATCAAAGTTAA
GAGCTATTCAGCCAAGAAACCGAAATCACAGAGAAGTGCTTGAAGAGCATTTACTAAAGG
CTGATGATGCTAATGACATTTCCTCTGAACTTCAACTCCAATGTCTGAATGTCTGATCTC
TCTCCCACAAGCAAGTGTTTGCAATCTGACAATTCCC
Primers used for PCR 55xo - LBb3, show primer details
   
Sequence Name 87-K119308-SALK_151204C-55xo
GenBank Accession LN486311 [GenBank]
Sequencing primer gene specific primer, 55xo: 5'-CCATAATATTTTGTGATTGTTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K119308-SALK_151204C-55xo
TGGGAAGCCTGCAAGGGTGGTAAAAAGTGTACTACTTGCCTTTAAACCATCTAAATCTAG
TGGTTCTAAAGATAAGGAGGTAACACACTTCCGCACAATGTTGTATGATAAAACAAATGA
ATTTCAAGCTTGCATAGTTAAATTTCTCAGGTTGATTACAGTAGTGGTTCTCGTAGAGGA
CCTTGACTGCTTGACATCTCTTTTACTAGTTTTTTCACTTTAGACATTGCAAATTTCCCA
CTAATTTTGTGTCGTCTACTATTGTCTATGGTGTCACTTTCTGATTTCATTTCCCTTTGT
TGTCTGTTTCTAATTGTTCCTGACAACAATTCTCTATGGTTTATCACGTGCAGAGAATTG
TGAATGGAAAAGAAGTAGTGGTTATATCAAAGATTGAAGAATCCGATGTTGTTTCGGATC
TTTCATCAATTGGAAATGCAGCAGTCTATACCAGTGGTATTGTAGAGACGCAGAATCTAA
AACATGAAGATGTTTCGGACGACGAGATACAAGTTTCTGAGGTCCAACCAACAGATTCTC
AAGATGTTGCTTCTGTTCCTGATGATTCGCTATCCGAATCAGAGAAAATTCAACAAGAGA
TCGCAGCAGTTACCGTGCAGGCTGCGTATAGAGGATACTTGGTAATATTCTTAAGCTTCT
GGACTAGATATAGACATGGCTCTTGATTTGCAGCTATTGGTGTTTTTCTTTTTCTACTTT
TCTTTTTTTCTATTCTGATATGGAGCTCTCTATGTGAAGGCACGACGTGCTTTTCAGATA
TTAAAAGGTATAAT
   
Sequence Name 39-K119307-SALK_151204C-R210
GenBank Accession LN485290 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K119307-SALK_151204C-R210
TCTACCAGGTGACCCGGATAAGTGCTTGTAGCCTTATTATACCTTTTAATATCTTGAAAG
CACGTCGTGCCTTCACATAGAGAGCTCATATCAGAATAGAAAAAAAGAAAAGTAGAAAAA
GAAAAACACCAATAGCTGCAAATCAAGAGCCATGTCTATATCTAGTCCAGAAGCTTAAGA
ATATTACCAAGTATCCTCTATACGCAGCCTGCACGGTAACTGCTGCGATCTCTTGTTGAA
TTTTCTCTGATTCGGATAGCGAATCATCAGGAACAGAAGCAACATCTTGAGAATCTGTTG
GTTGGACCTCAGAAACTTGTATCTCGTCGTCCGAAACATCTTCATGTTTTAGATTCTGCG
TCTCTACAATACCACTGGTATAGACTGCTGCATTTCCAATTGATGAAAGATCCGAAACAA
CATCGGATTCTTCAATCTTTGATATAACCACTACTTCTTTTCCATTCACAATTCTCTGCA
CGTGATAAACCATAGAGAATTGTTGTCAGGAACAATTAGAAACAGACAACAAAGGGAAAT
GAAATCAGAAAGTGACACCATAGACAATAGTAGACGACACAAAATTAGTGGGAAATTTGC
AATGTCTAAAGTGAAAAAACTAGTAAAAGAGATGTCAAGCAGTCAAGGTCCTCTACGAGA
ACCACTACTGTAATCAACCTGAGAAATTTAACTATGCAAGCTTGAAATTCATTTGTTTTA
TCATACAACATTGTGCGGAAGTGTGTTACCTCCTTATCTTTAGAACCACTAGATTTAGAT
GGTTTCTTTCCAAGTAGTACACTTTTTTACCACCTTGCAGGCTTTCCCATTATGTCCACA
ACCAAAATTCAAAACACCTGCA

Last Updated on 06.12.2018 16:11