Sequences from confirmation amplicon


Confirmation sequences for line SALK_151687c and hit At1g49910 are shown.
Line ID SALK_151687c
Locus At1g49910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18479795
Primers used for PCR 20db - LBb3, show primer details
   
Sequence Name 11-K109680-SALK_151687C-20db
GenBank Accession LN484578 [GenBank]
Sequencing primer gene specific primer, 20db: 5'-CTTCCCTCTTTCTCTCTGAGCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K109680-SALK_151687C-20db
TTGGTGCCGGCCATTGGTCGCGAGCTCTCGAATCCACCGTCCGATGGGATTTCTAATCTT
CGATTTTCTAATAACAGCGATCATTTACTAGTCTCTTCATGGGATAAGGTTAGAAATCTC
TAATCTTCTTTCAAACATTGATTTTACTGCTCCTTTGTAATTCTGATCTCTCTAATCTGA
TGGATTTTTGGATCGAAGAGTGTAAGATTGTATGATGCGAACGGCGATTTGATGAGAGGG
GAGTTTAAACATGGTGGAGCGGTACTCGATTGCTGCTTCCATGATGATTCTTCTGGATTC
AGTGTTTGCGCCGATACTAAAGTTAGAAGGTATGTTTAATTTGTTCTCTCTTCTAATTTT
ATCCGGTTCGATGCAATTTTCAAATTGAGATATAGTGAAACTGGTCATGTAGGAGTGTAC
AAATAGTGTTTAGGAATTGTTTTGCGGAATCTAAATAATTGGGTTTCTCTTGTTATACTT
CAGAATTGACTTCAATGCTGGCAAAGAAGACGTTTTGGGTACGCATGAGAAGCCAGTTCG
ATGTGTTGAGTATTCTTATGCTGCAGGTTGGTAAAAATTGGTTATGATGTTCGTGATGTT
TTGGTGTTTCTAATCATCCCATGGTTTTGCTTGAACACTATGTTTATGTGGCGTTATAGG
GCAAGTGATCACTGGAAGTTGGGATAAAACGATTAAATGTTGGGATCCAAGAGGTGCAAG
TGGGACTGAGCGCACACAG
   
Sequence Name 27-K109679-SALK_151687C-R210
GenBank Accession LN485056 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K109679-SALK_151687C-R210
GTCATTTGTTACCGCTCAGGTTGCATATAAGTTCCAATCTGTGTGCGCTCAGTCCCACTT
GCACCTCTTGGATCCCAACATTTAATCGTTTTATCCCAACTTCCAGTGATCACTTGCCCT
ATAACGCCACATAAACATAGTGTTCAAGCAAAACCATGGGATGATTAGAAACACCAAAAC
ATCACGAACATCATAACCAATTTTTACCAACCTGCAGCATAAGAATACTCAACACATCGA
ACTGGCTTCTCATGCGTACCCAAAACGTCTTCTTTGCCAGCATTGAAGTCAATTCTGAAG
TATAACAAGAGAAACCCAATTATTTAGATTCCGCAAAACAATTCCTAAACACTATTTGTA
CACTCCTACATGACCAGTTTCACTATATCTCAATTTGAAAATTGCATCGAACCGGATAAA
ATTAGAAGAGAGAACAAATTAAACATACCTTCTAACTTTAGTATCGGCGCAAACACTGAA
TCCAGAAGAATCATCATGGAAGCAGCAATCGAGTACCGCTCCACCATGTTTAAACTCCCC
TCTCATCAAATCGCCGTTCGCATCATACAATCTTACACTCTTCGATCCAAAAATCCATCA
GATTAGAGAGATCAGAATTACCAAGGAGCAGTAAAATCCATGTTTGAAAGAAGATTAGAG
AATTCTAACCTTATCCCATGAAGAGACTAGTAAATGATCGCTGTTATTAGAAAAT

Last Updated on 06.12.2018 16:11