Sequences from confirmation amplicon


Confirmation sequences for line SALK_151769c and hit At1g19370 are shown.
Line ID SALK_151769c
Locus At1g19370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6694744
Primers used for PCR 50sf - LBb3, show primer details
   
Sequence Name 50-K116586-SALK_151769C-50sf
GenBank Accession LN485515 [GenBank]
Sequencing primer gene specific primer, 50sf: 5'-TCTCTCTTTCTCTTCTCCGATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K116586-SALK_151769C-50sf
GAGTTGCAGATACTTAGCATCTAGATTAGCGACTAATTCCGTCAGATTCTTAACGAGTTC
CTCTAAATTATGCAATAGAACAGCTTCTCGATTGGTTTCGCTAACAGACACAGCAACATC
GGTAGAAACATCTTCGATAACCGGAGACTCGGTGGCATACAACGGCGAAATGAAAAGCAG
AGTGAAGGAGAATAAAAGGGAGAAACAGATAAAGAAGGACTTGCCGTTTAGAGGATCCGC
CATTACAGTAATGGGAAAAGTTAAAGCAGGATATATTGTGGTGTAAACAAATTGACGCTT
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAA
GCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 33-K116585-SALK_151769C-R210
GenBank Accession LN485189 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116585-SALK_151769C-R210
CTTTAACTTTTTCCATTACTGTAATGGCGGATCCTCTAAACGGCAAGTCCTTCTTTATCT
GTTTCTCCCTTTTATTCTCCTTCACTCTGCTTTTCATTTCGCCGTTGTATGCCACCGAGT
CTCCGGTTATCGAAGATGTTTCTACCGATGTTGCTGTGTCTGTTAGCGAAACCAATCGAG
AAGCTGTTCTATTGCATAATTTAGAGGAACTCGTTAAGAATCTGACGGAATTAGTCGCTA
ATCTAGATGCTAAGTTATCTGCAACTCCATTAAAGGAGAAGAACGAGATCTCAGTTGATG
ATGACATCGGA

Last Updated on 06.12.2018 16:11