SimpleSearch - Line and FST details
Line specific information
| Line ID | 044H05 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N404217) |
| Segregation Analysis | 50:43:35 |
| Confirmed for Hit | T12K4 At1g48520 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | none |
| Note |
Gene hit At5g66880
| Sequence (A. th genome BLAST matches underlined) | >40-K026600-022-044-H05-8409 CGGACAGAAGCCTTTACAATTGAATATATGCCGCCCTACATCGTCTCGTTACCAGAGTCT TGCTATTGTGGGAGATCTTTTTCCACTTATTTTCGCGAATCTTCGTCTTCTTCCTATGGG ATCCGCCCTATAGTGAGACTATGGGATCCTCCCTATAGCGAGTGGGATTACCCCCACCCG TGTGGGATCCTCCCTAGAGTGAGAATGGTTGCCGGCCCATATTGGGATGTCA |
| GenBank Accession | CR396050 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:26710541 - go to primer design |
| BLAST e Value | 9e-07 |
| Hit Clone Code (BAC ID) | MUD21 |
| Hit Gene Code | At5g66880 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | sucrose nonfermenting 1(SNF1)-related protein kinase 2.3 |
| Insertion Classification | TS2TE (5') |
| Confirmation Status | failed, line belongs to a contamination group, please check line 800C12 |
Gene hit At3g42624
| Sequence (A. th genome BLAST matches underlined) | >40-K015474-022-044-H05-8409 TAGTGAGAGGTGAACTACATGGAAACGTCATCGTCCTACTATCTTGAGTAGTAAGCAGGG GCCGAAGTTGAGGCGGGAGGATTTTGCTCGTTCTGTCCGGTCGAGCGTATGGAAGCTCGT CTCTATGGGATCCTCCCTATAGTGAGAAGAAAAAGAACGAGTAATTACCACTTTTTTTTC AACCCAAAAAAGTAAATATCCGCA |
| GenBank Accession | AL763967 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:14714633 - go to primer design |
| BLAST e Value | 9e-39 |
| Hit Clone Code (BAC ID) | T12K4 |
| Hit Gene Code | At3g42624 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | |
| Insertion Classification | TS2TE |
| Confirmation Status | confirmed, show confirmation sequences |
| Other FSTs Supporting this Hit | AL763967 [GenBank] |
Gene hit At1g48520
| Sequence (A. th genome BLAST matches underlined) | >49-011521-rosso-H5-35St CTTGAGTAAGGGATGACGCACAATNCCACTATCCTTCGCAAGACCCTTCCTCTATATAAG GAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCT TCGGTTTAAACTATCAGTGTTTAGTAAATAATGAACTTTGGAATTCAACAACTTTGGAAT TCAAAACAGGCAAAGCACCAGGCAAACCCATACAAACAGGACAAATACTTGTGTTTGGAT AAGAACCGTAATTGTTAGAGCAACTACAAAAGGCTTTTGTCAATGTAGAGAGCTGAACAT GAGTCTCTATCCCAATTACAGCTTCGTAATCTCTCAGAATCTCATCAAGTTGGTTGCTTT TGTGGTTTTTTGGAGCAACCCTTGTTGGAGAAGACTGTTTAGCTTCATGGGTCGTCGTTG TTTGGCTTCCACAAGACCTCACGGAGAAGTTGTTACTCCTTCTCCTTCTTAACAAGCTAG TTCCGAGAAGTGAGAACTGGTTTAGCTGAATTGTTCTCAACAATGTGGGATACATGTTTT AAGGTGGGATCTAATACTTTCGGGTTGAAAATTCCANTCCCCTCCTCATAACATAATTTA AAACATAAATA |
| GenBank Accession | AL763966 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr1:17940564 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | T1N15 |
| Hit Gene Code | At1g48520 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | GLU-ADT subunit B |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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