Genotyping and primer details


Genotyping and primer details for 676F05 - At3g57830
Line ID 676F05
Locus At3g57830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21421022 of line 676F05 Chr3:21421022
   
Insertion allele amplicon 8474 - DM20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DM20: 5'-CTCTCTTAAACCAAGGACCAACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21420406
   
wt amplicon (over InsPos) 48rd - DM20
wt amplicon start Chr3:21420406
wt amplicon end Chr3:21421371
wt amplicon size 966 bp
Locus specific primer DM20: 5'-CTCTCTTAAACCAAGGACCAACAG-3'
2nd locus specific primer 48rd: 5'-TGGAACCATAACAGTTACCAAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11