Genotyping and primer details


Genotyping and primer details for 410E04 - At5g19025
Line ID 410E04
Locus At5g19025 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6355424 of line 410E04 Chr5:6355424
   
Insertion allele amplicon 8474 - RK96
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK96: 5'-GAGATCATGAGAGCAACAAAGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6354831
   
wt amplicon (over InsPos) 04sl - RK96
wt amplicon start Chr5:6354831
wt amplicon end Chr5:6355950
wt amplicon size 1120 bp
Locus specific primer RK96: 5'-GAGATCATGAGAGCAACAAAGCTA-3'
2nd locus specific primer 04sl: 5'-TTTTCAGCACATTGAATCTTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11