Genotyping and primer details


Genotyping and primer details for 011D02 - At1g23010
Line ID 011D02
Locus At1g23010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8148991 of line 011D02 Chr1:8148991
   
Insertion allele amplicon 3144 - 45kb
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 45kb: 5'-ATAATCGTTAATGGAAAAGCATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8148497
   
wt amplicon (over InsPos) 34sq - 45kb
wt amplicon start Chr1:8148497
wt amplicon end Chr1:8149557
wt amplicon size 1061 bp
Locus specific primer 45kb: 5'-ATAATCGTTAATGGAAAAGCATGG-3'
2nd locus specific primer 34sq: 5'-TCATCATCATATTGTCCTCATGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11