Genotyping and primer details


Genotyping and primer details for SALK_047438c - At1g59900
Line ID SALK_047438c
Locus At1g59900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22051331 of line SALK_047438c Chr1:22051331
   
Insertion allele amplicon LBb3 - 55cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55cs: 5'-AGCAATACCACAACCTAATGGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22051901
   
wt amplicon (over InsPos) 28dw - 55cs
wt amplicon start Chr1:22050937
wt amplicon end Chr1:22051901
wt amplicon size 965 bp
Locus specific primer 55cs: 5'-AGCAATACCACAACCTAATGGAAC-3'
2nd locus specific primer 28dw: 5'-CGCCAGTTTTGCTGTATAGTGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11