Genotyping and primer details


Genotyping and primer details for 266D11 - At1g66470
Line ID 266D11
Locus At1g66470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24796570 of line 266D11 Chr1:24796570
   
Insertion allele amplicon 8474 - 18nz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18nz: 5'-CATTAAAATAAAACTCAATCTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24796088
   
wt amplicon (over InsPos) 01su - 18nz
wt amplicon start Chr1:24796088
wt amplicon end Chr1:24796870
wt amplicon size 783 bp
Locus specific primer 18nz: 5'-CATTAAAATAAAACTCAATCTCTC-3'
2nd locus specific primer 01su: 5'-ACTAGTTAAAGATTCCATCCCCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11