Genotyping and primer details


Genotyping and primer details for 290H06 - At1g66530
Line ID 290H06
Locus At1g66530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24820970 of line 290H06 Chr1:24820970
   
Insertion allele amplicon 8474 - 15fx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15fx: 5'-AGGCATAGTTAAAACCACCATCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24820587
   
wt amplicon (over InsPos) 12gs - 15fx
wt amplicon start Chr1:24820587
wt amplicon end Chr1:24821356
wt amplicon size 770 bp
Locus specific primer 15fx: 5'-AGGCATAGTTAAAACCACCATCAC-3'
2nd locus specific primer 12gs: 5'-ATTCATTGAAGTAAAAAGCTGCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11