Genotyping and primer details


Genotyping and primer details for 731E02 - At1g70340
Line ID 731E02
Locus At1g70340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26505142 of line 731E02 Chr1:26505142
   
Insertion allele amplicon 8474 - 29ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29ve: 5'-TCTCCTTTGCTAACCTAATCGTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26504809
   
wt amplicon (over InsPos) 40vn - 29ve
wt amplicon start Chr1:26504809
wt amplicon end Chr1:26505527
wt amplicon size 719 bp
Locus specific primer 29ve: 5'-TCTCCTTTGCTAACCTAATCGTGT-3'
2nd locus specific primer 40vn: 5'-CCCGAGAAAGATACCAATTTATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11