Genotyping and primer details


Genotyping and primer details for 294C01 - At2g02820
Line ID 294C01
Locus At2g02820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:805978 of line 294C01 Chr2:805978
   
Insertion allele amplicon 8474 - 13us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13us: 5'-CAATGAACTGAGAAGTTCAGCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:805671
   
wt amplicon (over InsPos) 46vc - 13us
wt amplicon start Chr2:805671
wt amplicon end Chr2:806336
wt amplicon size 666 bp
Locus specific primer 13us: 5'-CAATGAACTGAGAAGTTCAGCTTG-3'
2nd locus specific primer 46vc: 5'-GTGTAGAACGGATAATGCTGTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11