Genotyping and primer details


Genotyping and primer details for 509D03 - At2g22870
Line ID 509D03
Locus At2g22870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9739761 of line 509D03 Chr2:9739761
   
Insertion allele amplicon 3144 - 61xd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 61xd: 5'-TATTTTTGAAAAGCTCCCACTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9738688
   
Insertion allele amplicon 8474 - CC77
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC77: 5'-CAGAACTAAGCCAATCGAACTACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9740237
   
wt amplicon (over InsPos) AP34 - CC77
wt amplicon start Chr2:9739259
wt amplicon end Chr2:9740237
wt amplicon size 979 bp
Locus specific primer CC77: 5'-CAGAACTAAGCCAATCGAACTACC-3'
2nd locus specific primer AP34: 5'-AAAAGTTGCGCTAGATCAAAACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11