Genotyping and primer details


Genotyping and primer details for 840E05 - At2g25010
Line ID 840E05
Locus At2g25010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10632740 of line 840E05 Chr2:10632740
   
Insertion allele amplicon 8474 - NS47
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NS47: 5'-TGCTTCTTTCGATGTTGTTAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10632264
   
wt amplicon (over InsPos) 24si - NS47
wt amplicon start Chr2:10632264
wt amplicon end Chr2:10633144
wt amplicon size 881 bp
Locus specific primer NS47: 5'-TGCTTCTTTCGATGTTGTTAAATG-3'
2nd locus specific primer 24si: 5'-ATACCATTCCATGTAAGCTCCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11