Genotyping and primer details


Genotyping and primer details for 915D10 - At2g42380
Line ID 915D10
Locus At2g42380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17648666 of line 915D10 Chr2:17648666
   
Insertion allele amplicon 8474 - 18nw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18nw: 5'-ATGTGGCCCTACTCAGAGAGATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17649138
   
wt amplicon (over InsPos) 71rd - 18nw
wt amplicon start Chr2:17648251
wt amplicon end Chr2:17649138
wt amplicon size 888 bp
Locus specific primer 18nw: 5'-ATGTGGCCCTACTCAGAGAGATAA-3'
2nd locus specific primer 71rd: 5'-TTGTTATCGTCGTTGAAGCTATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11