Genotyping and primer details


Genotyping and primer details for 309F08 - At3g27570
Line ID 309F08
Locus At3g27570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10215277 of line 309F08 Chr3:10215277
   
Insertion allele amplicon 8474 - 59wd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 59wd: 5'-TTCTTTGCGTTGTTATGGATACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10215911
   
Insertion allele amplicon 8474 - LG04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer LG04: 5'-TTGAAAACACAGCGTTAAATGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10214631
   
wt amplicon (over InsPos) LG05 - LG04
wt amplicon start Chr3:10214631
wt amplicon end Chr3:10215772
wt amplicon size 1142 bp
Locus specific primer LG04: 5'-TTGAAAACACAGCGTTAAATGAAG-3'
2nd locus specific primer LG05: 5'-TTCTTGCTTCGTATTTCGATTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11