Genotyping and primer details


Genotyping and primer details for 537G03 - At4g02610
Line ID 537G03
Locus At4g02610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1148434 of line 537G03 Chr4:1148434
   
Insertion allele amplicon 8474 - 64ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 64ra: 5'-CTCTCTTTCTTGTCATAGGCTGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1148033
   
wt amplicon (over InsPos) 28rt - 64ra
wt amplicon start Chr4:1148033
wt amplicon end Chr4:1148824
wt amplicon size 792 bp
Locus specific primer 64ra: 5'-CTCTCTTTCTTGTCATAGGCTGCT-3'
2nd locus specific primer 28rt: 5'-TAAGAACACGAACTTACACGCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11