Genotyping and primer details


Genotyping and primer details for 358F09 - At4g02660
Line ID 358F09
Locus At4g02660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1174239 of line 358F09 Chr4:1174239
   
Insertion allele amplicon 8474 - HM35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HM35: 5'-TCTCGGCTTTTCAAGGAGTAAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1173515
   
wt amplicon (over InsPos) 07tf - HM35
wt amplicon start Chr4:1173515
wt amplicon end Chr4:1174626
wt amplicon size 1112 bp
Locus specific primer HM35: 5'-TCTCGGCTTTTCAAGGAGTAAATA-3'
2nd locus specific primer 07tf: 5'-CACTCAAGCAGTCAAGCTATTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11