Genotyping and primer details


Genotyping and primer details for 114D03 - At4g26300
Line ID 114D03
Locus At4g26300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13309063 of line 114D03 Chr4:13309063
   
Insertion allele amplicon 8474 - 06fx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06fx: 5'-CAATTATGATGAATGATTTGCCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13309604
   
wt amplicon (over InsPos) 14gs - 06fx
wt amplicon start Chr4:13308547
wt amplicon end Chr4:13309604
wt amplicon size 1058 bp
Locus specific primer 06fx: 5'-CAATTATGATGAATGATTTGCCAG-3'
2nd locus specific primer 14gs: 5'-TAGTGGTTGATTATGCAGCTATGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11