Genotyping and primer details


Genotyping and primer details for 252G10 - At5g02320
Line ID 252G10
Locus At5g02320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:483867 of line 252G10 Chr5:483867
   
Insertion allele amplicon 8474 - RS03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RS03: 5'-ACCTGTACTTTTTGCAGGACTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:484476
   
wt amplicon (over InsPos) 01si - RS03
wt amplicon start Chr5:483581
wt amplicon end Chr5:484476
wt amplicon size 896 bp
Locus specific primer RS03: 5'-ACCTGTACTTTTTGCAGGACTGAT-3'
2nd locus specific primer 01si: 5'-GCTGTTCTTTTAGCTCTTAACCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11