Genotyping and primer details


Genotyping and primer details for 196B10 - At5g53200
Line ID 196B10
Locus At5g53200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21583872 of line 196B10 Chr5:21583872
   
Insertion allele amplicon 8474 - BL82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BL82: 5'-CGATATACCGCGGATTAAAATCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16905839
   
wt amplicon (over InsPos) 19fo - BL82
wt amplicon start Chr1:16905839
wt amplicon end Chr5:21583509
wt amplicon size 693 bp
Locus specific primer BL82: 5'-CGATATACCGCGGATTAAAATCTA-3'
2nd locus specific primer 19fo: 5'-TACAGTGTACCATGTTGTAGGGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11