Genotyping and primer details


Genotyping and primer details for 013E09 - At1g05570
Line ID 013E09
Locus At1g05570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1650909 of line 013E09 Chr1:1650909
   
Insertion allele amplicon 8474 - PD19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PD19: 5'-TTTGAAAGCTTCCTCCATGTAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1650308
   
Insertion allele amplicon 8474 - VL36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer VL36: 5'-TAGACAAAAGAAAACTACGCATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1650430
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11