Genotyping and primer details


Genotyping and primer details for 049C01 - At5g02070
Line ID 049C01
Locus At5g02070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:406836 of line 049C01 Chr5:406836
   
Insertion allele amplicon 8474 - DL59
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DL59: 5'-CTTGTTGGGTAAATCAAAATGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:407314
   
Insertion allele amplicon 3144 - FM04
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FM04: 5'-CTCTCGTTATTAGCGGTTTCTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:406295
   
wt amplicon (over InsPos) DL59 - FM04
wt amplicon start Chr5:406295
wt amplicon end Chr5:407314
wt amplicon size 1020 bp
Locus specific primer FM04: 5'-CTCTCGTTATTAGCGGTTTCTGTC-3'
2nd locus specific primer DL59: 5'-CTTGTTGGGTAAATCAAAATGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11