Genotyping and primer details


Genotyping and primer details for 156C08 - At4g27595
Line ID 156C08
Locus At4g27595 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13776403 of line 156C08 Chr4:13776403
   
Insertion allele amplicon 8474 - 07of
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07of: 5'-TCTAGACTGAGATGGGATCCTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13776678
   
wt amplicon (over InsPos) 39sq - 07of
wt amplicon start Chr4:13775942
wt amplicon end Chr4:13776678
wt amplicon size 737 bp
Locus specific primer 07of: 5'-TCTAGACTGAGATGGGATCCTGTT-3'
2nd locus specific primer 39sq: 5'-CTTCCAAGAAACTTCCTTTTTGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11