Genotyping and primer details


Genotyping and primer details for 257A11 - At5g11050
Line ID 257A11
Locus At5g11050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3503323 of line 257A11 Chr5:3503323
   
Insertion allele amplicon 8474 - RS04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RS04: 5'-GTAGCTAGAACAATGGGAACCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3503639
   
wt amplicon (over InsPos) 16fo - RS04
wt amplicon start Chr5:3503028
wt amplicon end Chr5:3503639
wt amplicon size 612 bp
Locus specific primer RS04: 5'-GTAGCTAGAACAATGGGAACCAAA-3'
2nd locus specific primer 16fo: 5'-ATATTGATGATCTCTCTCCATCCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11