Genotyping and primer details


Genotyping and primer details for 289B11 - At1g77760
Line ID 289B11
Locus At1g77760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29238171 of line 289B11 Chr1:29238171
   
Insertion allele amplicon 8474 - BE52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE52: 5'-AGATCAAGAACCTCAACGTCAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29237852
   
wt amplicon (over InsPos) 05tf - BE52
wt amplicon start Chr1:29237852
wt amplicon end Chr1:29238435
wt amplicon size 584 bp
Locus specific primer BE52: 5'-AGATCAAGAACCTCAACGTCAAGT-3'
2nd locus specific primer 05tf: 5'-TCGGATGGTTAAATGGTTAAAGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11