Genotyping and primer details


Genotyping and primer details for 687C03 - At2g37130
Line ID 687C03
Locus At2g37130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15598239 of line 687C03 Chr2:15598239
   
Insertion allele amplicon 8474 - AP29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP29: 5'-GTTTGGTATTTGATTCGGAGATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15598831
   
wt amplicon (over InsPos) AP28 - AP29
wt amplicon start Chr2:15597692
wt amplicon end Chr2:15598831
wt amplicon size 1140 bp
Locus specific primer AP29: 5'-GTTTGGTATTTGATTCGGAGATGT-3'
2nd locus specific primer AP28: 5'-TGATTGTGAAAAGAGTCAATGGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11