Genotyping and primer details


Genotyping and primer details for 853D02 - At2g35710
Line ID 853D02
Locus At2g35710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15014048 of line 853D02 Chr2:15014048
   
Insertion allele amplicon 8474 - 33iv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 33iv: 5'-AGAGACTTACAAGGTTTGAACCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15013717
   
wt amplicon (over InsPos) 04jp - 33iv
wt amplicon start Chr2:15013717
wt amplicon end Chr2:15014596
wt amplicon size 880 bp
Locus specific primer 33iv: 5'-AGAGACTTACAAGGTTTGAACCCA-3'
2nd locus specific primer 04jp: 5'-TAGTGCGTTACAAACTAACAGCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11