Genotyping and primer details


Genotyping and primer details for SALK_025237c - At1g15020
Line ID SALK_025237c
Locus At1g15020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5175583 of line SALK_025237c Chr1:5175583
   
Insertion allele amplicon LBb3 - 37wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37wm: 5'-TGATCCAGTTCAACAAAAGATCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5175116
   
wt amplicon (over InsPos) 27xa - 37wm
wt amplicon start Chr1:5175116
wt amplicon end Chr1:5176280
wt amplicon size 1165 bp
Locus specific primer 37wm: 5'-TGATCCAGTTCAACAAAAGATCAG-3'
2nd locus specific primer 27xa: 5'-AGTATGAAAGTTGTCGGAGGAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11