Sequences from confirmation amplicon


Confirmation sequences for line SALK_025237c and hit At1g15020 are shown.
Line ID SALK_025237c
Locus At1g15020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5175583
Primers used for PCR 37wm - LBb3, show primer details
   
Sequence Name 37-K118709-SALK_025237C-37wm
GenBank Accession LN485258 [GenBank]
Sequencing primer gene specific primer, 37wm: 5'-TGATCCAGTTCAACAAAAGATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118709-SALK_025237C-37wm
TTTTTCTCTTGTTAGGTCCCCAGCTGCCACCAACGAACCTTTTAGGAGGAGCCCAAAAGA
GCATTGGATAATGATTGATAGAGAACTTGTCACAAAGCTTTACATTCATCTGTGCTGCCA
GAAAAGTCACAATAACAATTAGAAAGAAAAAAAACTATACACAACCTCATAAGATACAGT
TTTCAAGCCCCACGCTCTGACTCACACGATCCACATGAAGTAAGAGAAAAAAGAGAAAGG
AAGGTCAAGATACACGTTCATCTATATAAATAAAAAAGATGGTGCAAGTACCTTTATCGC
ACAATCAACTCTGGTCATCAAAACGACACCAGGATATACTGCGTCTGCTCCATTGAATAG
CCTTGCAACTTTTTCATAATGTGGCTGCAGCAGAGTCACCCATTGGATATATTGTGGTGT
AAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCT
GAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGA
TGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 12-K118708-SALK_025237C-R210
GenBank Accession LN484628 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118708-SALK_025237C-R210
CATTTGTTTACCACAATATATCCAATGGGTGACTCTGCTGCAGCCACATTATGAAAAAGT
TGCAAGGCTATTCAATGGAGCAGACGCAGTATATCCTGGTGTCGTTTTGATGACCAGAGT
TGATTGTGCGATAAAGGTACTTGCACCATCTTTTTTATTTATATAGATGAACGTGTATCT
TGACCTTCCTTTCTCTTTTTTCTCTTACTTCATGTGGATCGTGTGAGTCAGAGCGTGGGG
CTTGAAAACTGTATCTTATGAGGTTGTGTATAGTTTTTTTTCTTTCTAATTGTTATTGTG
ACTTTTCTGGCAGCACAGATGAATGTAAAGCTTTGTGACAAGTTCTCTATCAATCATTAT
CCAATGCTCTTTTGGGCTCCTCCTAAAAGGTTCGTTGGTGGCAGCTGGGGACCTAAACAA
GAGAAAAATGAGATAAGCGTAGTCAATGAATGGCGCACTGCTGATCTTTTGTTGAACTGG
ATC

Last Updated on 06.12.2018 16:11