Sequences from confirmation amplicon


Confirmation sequences for line 054D08 and hit At1g14110 are shown.
Line ID 054D08
Locus At1g14110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4830530
Primers used for PCR MP08 - 8474, show primer details
   
Sequence Name 05-K018611-022-054-D08C-MP08
GenBank Accession LN488157 [GenBank]
Sequencing primer gene specific primer, MP08: 5'-AGCCAAGTGACTTTATCAAGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K018611-022-054-D08C-MP08
CTTGATCTTCTTCCAGAAGAACTTTTTGTCATGATCCCTGGAATCATGGAAAATATCAAG
ATAAAGATGTGACGGTATTATTGAAGTTGAGTTAATGCCATGATTCTTCAACATTGTTCC
GTAACATCGCGAGTGTTCCCTGTTGAAGCTATCTAACTGATCATTCAATGGAAAATCTAG
AGGAAGTAACCAGGAAGTTTCGGGGAAAGGCTCGCAGAAGAGGTCAGTTATATCGTTCCG
TTGGTCAACAAGCATGACTCTATCTGTCAAGAGAGCATAGAGGAAGACAGAAACCAGAGA
AAGCATTCTGTTTCCTAGACCAAACATAGGCATCCACACTACATACCGACATTCATCACC
AGAGCTGCTTAATTCATTGTGACCAAGCTGCTTTGTAGCTCTCTTGTAAGCTTTTGTGCC
AGGCCCGCAACGCTTGTGAAGCATCTCGTAGCTTCTAAGCTTAGAAACAATATGTCTAGA
TGGTTTGTATGGTGAAGGCTTGCTCATAGACTGATCATACCTACTTAAGCAAGATTTCTC
TTCAANACCCTGTAGCAGGATACACACGTATGTCAAATTCATGGCCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAATG
   
Sequence Name 06-K018611-022-054-D08C-8409
GenBank Accession LN488603 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018611-022-054-D08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGCCATGAATTTGACAT
ACGTGTGTATCCTTGCTACAGGGTTTGAAGAGAAATCTTGCTTAAGTAGGTATGATCAGT
CTATGAGCAAGCCTTCACCATACAAACCATCTAGACATATTGTTTCTAAGCTTAGAAGCT
ACGAGATGCTTCACAAGCGTTGCGGGCCTGGCACAAAAGCTTACAAGAGAGCTACAAAGC
AGCTTGGTCACAATGAATTAAGCAGCTCTGGTGATGAATGTCGGTATGTAGTGTGGATGC
CTATGTTTGGTCTAGGAAACAGAATGCTTTCTCTGGTTTCTGTCTTCCTCTATGCTCTCT
TGACAGATAGAGTCATGCTTGTTGACCAACGGAACGATATAACTGACCTCTTCTGCGAGC
CTTTCCCCGAAACTTCCTGGTTACTTCCTCTAGATTTTCCATTGAATGATCAGTTAGATA
GCTTCAACAGGGAACACTCGCGATGTTACGGAACAATGTTGAAGAATCATGGCATTAACT
CAACTTCAATAATACCGTCACATCTTTATCTTGATATTTTCCATGGATTCCGGGATCATG
ACAAAAAGTTCTTCTGTGAAGAAGATCAAGCTTTCCTTGA

Last Updated on 06.12.2018 16:11