Sequences from confirmation amplicon


Confirmation sequences for line 460F06 and hit At2g04038 are shown.
Line ID 460F06
Locus At2g04038 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1332188
Primers used for PCR NU28 - 8474, show primer details
   
Sequence Name 47-K103927-0022-460-F06C-NU28
GenBank Accession LN506184 [GenBank]
Sequencing primer gene specific primer, NU28: 5'-TTTGTCCATGTTCCACCACTATAA-3'
Sequence (A. th genome BLAST matches underlined)
>47-K103927-0022-460-F06C-NU28
TTTTCTTCTTATTCTATGCTCTTATAATCCATAGGACCCACTTCTACATGATTCTCCTAC
AACCCCATTATAAAACCCTTGGTCCACTTCCTCCTCTTACTTCAAAACTAACTTCCCAAT
TCTTCACACAAAATAAAAACACAGAAACTCTTACATACTCATATAAACCAAACTAAAACC
ATGATTCCGGCAGAAATCAACGGATATTTCCAATATCTATCACCGGAATACAACGTAATA
AACATGCCTTCATCTCCAACCTCTTCCTTAAACTACCTAAACGATTTGATCATCAACAAC
AACAACTATTCCTCATCATCCAACAGTCAAGATCTCATGATAAGCAACAACTCAACTTCC
GACGAAGATCATCATCAAAGCATCATGGTACTCGACGAGAGGAAACAGAGAAGGATGCTT
TCGAACAGAGAAATCTGCAAGGAGGTCAAGGGACATCTTACATGGAGTCAACAATGAGCA
TGAT
   
Sequence Name 63-K103928-0022-460-F06C-8409
GenBank Accession LN510977 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>63-K103928-0022-460-F06C-8409
AGATTCTCTGTTCGAAGCATCCTTCTCTGTTTCCTCTCGTCGAGTACCATGATGCTTTGA
TGATGATCTTCGTCGGAAGTTGAGTTGTTGCTTATCATGAGATCTTGACTGTTGGATGAT
GAGGAATAGTTGTTGTTGTTGATGATNAAATCGTTTATGTAGTTTAAGGA

Last Updated on 06.12.2018 16:11