Sequences from confirmation amplicon


Confirmation sequences for line 165F12 and hit At2g40370 are shown.
Line ID 165F12
Locus At2g40370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16860873
Primers used for PCR KC77 - 8474, show primer details
   
Sequence Name 03-K103100-0022-165-F12C-KC77
GenBank Accession LN487443 [GenBank]
Sequencing primer gene specific primer, KC77: 5'-ATCGGTAAAATTAGGAGGGCTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103100-0022-165-F12C-KC77
GACCGAACGNTCAAGCAGGATCAAGATCCAATTAATAACGAAAGCGACTAGCATAATTAT
TGATTAGCCATTATCAAAACGATCTTATCTCAAGCCTTATGTATTAAATGTCAATTAAAG
CTGTCCAATCTTAGAGAATTATAGTGTCCAATCTTGGGGAATATTTACATGTTGTGCATC
CGTGCATGCAGGTCAATTACATGAGCATTTGATATTTATTTAGATAGAGATCACACAACA
TTGCGTACTTAAATTTTGTTGGCAACCCAGTGTACACATCTCCAGATACTATTTTGATCC
ATTTAATGGAGGGTCCAAAACAAAATCTTATATTAGTGTATAATTTATATGTACTATGTT
TATGGCAATTATGGAATATTACATCAAAAGTCAATAGAAAAAGCGTGTTAACCAAGAATT
CAAGAGACGTAATCACCTTTCCAATTTTATTTGTTAACTCGGTGATTTTTTTTGTTTTTC
TTATAGGCCCCAGTGGGTGCAACTACATGGATCAGCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 03-K103101-0022-165-F12C-8409
GenBank Accession LN487444 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103101-0022-165-F12C-8409
CATTTACAATTGCTGAATCCATGTAGTTGCACCCACTGGGGCCTATAAGAAAAACAAAAA
AAATCACCGAGTTAACAAATAAAATTGGAAAGGTGATTACGTCTCTTGAATTCTTGGTTA
ACACGCTTTTTCTATTGACTTTTGATGTAATATTCCATAATTGCCATAAACATAGTACAT
ATAAATTATACACTAATATAAGATTTTGTTTTGGACCCTCCATTAAATGGATCAAAATAG
TATCTGGAGATGTGTACACTGGGTTGCCAACAAAATTTAAGTACGCAATGTTGTGTGATC
TCTATCTAAATAAATATCAAATGCTCATGTAATTGACCTGCATGCACGGATGCACAACAT
GTAAATATTCCCCAAGATTGGACACTATAATTCTCTAAGATTGGACAGCTTTAATTGACA
TTTAATACATAAGGCTTGAGATAAGATCGTTTTGATAATGGCTAATCAATAATTATGCTA
GTCGCTTTCGTTATTAATTGGATCTTGATCCTGCTTGGACTGTTCGGTCTTGGTATAAGA
ACTGTGTTTTTCTACGGTCTTTAGCCCTCCTATTTTTACCG

Last Updated on 06.12.2018 16:11