Sequences from confirmation amplicon


Confirmation sequences for line 511C01 and hit At3g61470 are shown.
Line ID 511C01
Locus At3g61470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22745607
Primers used for PCR ZZ56 - 8474, show primer details
   
Sequence Name 05-K021642-022-511-C01C-8409
GenBank Accession LN488168 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K021642-022-511-C01C-8409
CATTTACAATTGAATATATGAAGAAAAAGAAAGACAACAACACAACACACGTAGGGATAA
TCAGATAAGCAGAATCTGATTTTGTCACCAGAGTGCTTTTATAGATTACACAACACACGT
TCGTTTCTTTCTTCTTCTAACACACTCTGAGTCTTGAAGGTCAACAACGTCGAGAATAAT
GGCATCATCTCTTTGTGCTTCTTCTGCCATCGCTGCCATTTCTTCTCCAAGGTAAGCTCA
TCTTTTTCTCTCTTCTTATCAGTTATCATCACTTGCTATGTAGGAATGAGACTAAGATCC
TTGTACAGTTGTACTTAATTAGTTTCGTTAACATGACTATGATAGTGATCCATTGATTGG
GATTTATGATCTAGTATCCGAGAATGTGTGTGCGGTTGTAAAATTAGTGATCCATCATCC
ATTTAGATTAAACGTTGTGCGACTATTGTTTGTGTGATCCAAACACCGATCTGCCCTATG
TAATAATATGAATTTATGCATGGTTGGTGTTATTGAAGTTGTGACTAAATTTTTAGGGCT
ATGATATATGTGCAGTTTCTTGGGTGGTAAGAAACTGAGGCTGAAGAAGAAGTTGACGTT
TTCCAG
   
Sequence Name 06-K021642-022-511-C01C-ZZ56
GenBank Accession LN488615 [GenBank]
Sequencing primer gene specific primer, ZZ56: 5'-AGCTGGAACAGTCAACTTCTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K021642-022-511-C01C-ZZ56
CCAGAACTGCACATATATCATAGCCCTAAAAATTTAGTCACAACTTCAATAACACCAACC
ATGCATAAATTCATATTATTACATAGGGCAGATCGGTGTTTGGATCACACAAACAATAGT
CGCACAACGTTTAATCTAAATGGATGATGGATCACTAATTTTACAACCGCACACACATTC
TCGGATACTAGATCATAAATCCCAATCAATGGATCACTATCATAGTCATGTTAACGAAAC
TAATTAAGTACAACTGTACAAGGATCTTAGTCTCATTCCTACATAGCAAGTGATGATAAC
TGATAAGAAGAGAGAAAAAGATGAGCTTACCTTGGAGAAGAAATGGCAGCGATGGCAGAA
GAAGCACAAAGAGATGATGCCATTATTCTCGACGTTGTTGACCTTCAAGACTCAGAGTGT
GTTAGAAGAAGAAAGAAACGAACGTGTGTTGTGTAATCTATAAAAGCACTCTGGTGACAA
AATCAGATTCTGCTTATCTGATTATCCCTACGTGTGTTGTGTTGTTGTCTTTCTTTTTCT
TCATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACA

Last Updated on 06.12.2018 16:11