Sequences from confirmation amplicon


Confirmation sequences for line 138H01 and hit At4g11230 are shown.
Line ID 138H01
Locus At4g11230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6845631
Primers used for PCR ME47 - 3144, show primer details
   
Sequence Name 06-K027636-022-138-H01C-ME47
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, ME47: 5'-AGGTCTAGTTGGTCGCTGAGATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K027636-022-138-H01C-ME47
GAATTTGTTAACTCACAATTGTCATTAACACCATATCGAATGCAGATTTAGTTCTGCTTA
CTTCTTGTACTCAATTCAATTCACAACTAGAAACAGATCCAGAATTTTCCTACCACTGAA
CAGAAAACAACGTGTTAAGAGGTTAAATGACGAACACAACAGAAAATA
   
Sequence Name 05-K027636-022-138-H01C-3144
GenBank Accession LN488211 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K027636-022-138-H01C-3144
CAATCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGG
ACAGGGTACCCGGGGATCAGATTGTCATTTTCCGGCGGGACTCACAACGATCGTTGGGGC
TCCGATTTAGCTTCCGCCGGAGAATTCACCCAAAGCTTTCCATCCTTACCAGCAACATAC
TCACCCTCACCCTCCTCCTCCTCCTCCTCCGGCGAAGAGCTCCTAGAAGTAACAATCGAA
TTCCCAAGCGGCGTAATAATAAACATCGACTCCGTTACCGGAACCGGCACCGATATTTCA
GGTACCGATCTAGAGATCACCTCTTGCTCGGATTCCGGTTCAGGATCAAGGTCGCTTAGC
TTAGGATGGAGCGCGTCGTCGGAGAGACTGACGGCGGGGACGAACTCAAAGCAGCAGATC
CAGAAGATCTCACGGCGTGGTTACGGTTATTCGTCTCGGTCTGCACCGGAACCGGTGGTT
CCACACAGAGGCGAAATTACCGATTCGGTTAATCTCCCTCGAGCTTTATCTCAG
Primers used for PCR FL15 - 8474, show primer details
   
Sequence Name 46-K028351-022-138-H01C-8409
GenBank Accession LN505754 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K028351-022-138-H01C-8409
AGACTGAATAAATACACGTTTTTGAATTTTGTCCTTTAAAGTAAAATTTGTNTTTTGTAC
TAATCAAAGGAACTACCAGAGGAAATTACCAAAGCAGAACGAAAATATAAAAATAAGTTT
GGTTTCTAAAAATACGGGTTTTAAATTATACTATATTTACGAGTTACGACACAAAAAATT
ATACTATATTTTTGTGCATGTTTTAGGTACACTTTAATTATACTTAACTCGCTGTTGTTG
TGAAATTATATATTTTTTTAAAAAAATTTAGTTTGCTTAGCATATTAAAAATATATATGA
TTTAATGTTTTGAATTTTTATATATTAATTAATGTATAATTATTATATTTGATTTTTATT
CTGTAAAATATAAAAACAATAAAATACAATTCATTTGGTTAAAATCTATATCACAACTAA
ATAATTTGAATTTGAATTGCATTTGTTTTGTTTAGTGTTCAAAACTAGAGGTATCAAAAT
GTCTCAACTCAACTCAATCC
   
Sequence Name 46-K028351-022-138-H01C-FL15
GenBank Accession LN505755 [GenBank]
Sequencing primer gene specific primer, FL15: 5'-TCATGGATTGAGTTGAGTTGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K028351-022-138-H01C-FL15
ATATAGATCTTAACCAAACGAATCGTATTTTATCGTTTTTATATTTTAAAGAATAAAAAT
CAAATATAATAATTATACATTAATTAATATATAAAAATTCAAAACATTAAATCATATATA
TTTTTAATATGCTAAACAAATTAAATTTTTTTAAAAAAATATATAATTTCACAACAACAG
CGAGTTAAGTATAATTAAAGTGTACCTAAAACATGCACAAAAATATAGTATAATTTTTTG
TGTCGTAACTCGTAAATATAGTATAATTTAAAACCGTATTTTTAGAAACCAAACTTATTT
TTATATTTTCGTTCTGCTTTGGTAATTTCCTCTGGTCGTTCCTTTGATTAGTACAAAAGA
CAAATTTTATAATAAACACAAAATTCAAAAACTGTATTTATTCAGTCTTCTCCCTCCTTG
GCTTCTCTCCTCTCCTCCCAACAAATTCAAAATGTCAATGTGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11