Sequences from confirmation amplicon


Confirmation sequences for line 588F03 and hit At5g56610 are shown.
Line ID 588F03
Locus At5g56610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22916734
Primers used for PCR NG20 - 8474, show primer details
   
Sequence Name 13-K102362-0022-588-F03C-8409
GenBank Accession LN491981 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K102362-0022-588-F03C-8409
TTACAATTGAATATATCCTGGCGATGACCGATGAAACGGAGGAAGATGACACGACGCAGC
AGCGATCATCACGAAACGACGGCGTTTCAAAGAATAAGGGTAAGGGTTTTAAGGGAGATA
AAGCAAAGAGGGCTCTAATCGGAGCTGGTGGTCGGATCCTTTTTTACCCAACTCTTTTGT
ACAACCTCGTCCGCTTTAAACTCCAGTCTCAATTCCGATGGTGGGATCAAATCGATGAGG
TTTCTTACTTATTTGTTATTTATTCCTGTTAACTTAGTGCTAATGGGATTTAAAGGAGAT
AACTTTTTAGCATTAGGAATCTGAATTTTGACTAGAATGTTTTGATCCGAAAGTGGAGGC
AATGTGATGTTTAAAGATCAGTTTTTTCAGACTCTTTAAAGTCAGAATTTTTTCTGTCTG
TATAGTTATGCAGTAGACTATCGAAATTTTGAGCTCTTTACTAAAATCTCTGAGTATCAA
TTTGGTAGTGATTTTGAGTCTATGCAGAAAGTCTGATACAGAAAGTCTCTATCTTTTCAG
TATCTTTTGATGGGAGCGGTTCCATTTCGTAAGGATGTTCCGAGACTAAAAAAGTTAGGT
GTTGGTGGGGTAATCACTCTTAATGAAC
   
Sequence Name 13-K102363-0022-588-F03C-NG20
GenBank Accession LN491982 [GenBank]
Sequencing primer gene specific primer, NG20: 5'-GGTTCATTAAGAGTGATTACCCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K102363-0022-588-F03C-NG20
CTTTTGTCTCGGACATCCTTACGAAATGGAACCGCTCCCATCAAAAGATACTGAAAAGAT
AGAGACTTTCTGTATCAGACTTTCTGCATAGACTCAAAATCACTACCAAATTGATACTCA
GAGATTTTAGTAAAGAGCTCAAAATTTCGATAGTCTACTGCATAACTATACAGACAGAAA
AAATTCTGACTTTAAAGAGTCTGAAAAAACTGATCTTTAAACATCACATTGCCTCCACTT
TCGGATCAAAACATTCTAGTCAAAATTCAGATTCCTAATGCTAAAAAGTTATCTCCTTTA
AATCCCATTAGCACTAAGTTAACAGGAATAAATAACAAATAAGTAAGAAACCTCATCGAT
TTGATCCCACCATCGGAATTGAGACTGGAGTTTAAAGCGGACGAGGTTGTACAAAAGAGT
TGGGTAAAAAAGGATCCGACCACCAGCTCCGATTAGAGCCCTCTTTGCTTTATCTCCCTT
AAAACCCTTACCCTTATTCTTTGAAACGCCGTCGTTTCGTGATGATCGCTGCTGCGTCGT
GTCATCTTCCTCCGTTTCATCGGTCATCGCCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGAGGGTCAATAT

Last Updated on 06.12.2018 16:11