Sequences from confirmation amplicon


Confirmation sequences for line 007C10 and hit At3g27810 are shown.
Line ID 007C10
Locus At3g27810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10308869
Primers used for PCR DR06 - 8474, show primer details
   
Sequence Name 25-K018967-022-007-C10C-DR06
GenBank Accession LN497235 [GenBank]
Sequencing primer gene specific primer, DR06: 5'-TTCAGTTGTGTCTGTCATGAGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K018967-022-007-C10C-DR06
ACTATTATTACATGTACAATGTTTGGATCTTTTTCAATTTCTAGATTATTTTTTTCTTTC
TTTCTGATAAGCCTTATAAATTATATAATTTATTTGTTGTTAAAACTTAAAAGCGTTACA
ATTTTTTGTTTGTTTGTTAAAAGTGTTAATAAACGGTACTTCTAAGATTAACTGATAAAA
GCTAGATGTCAATTAAACTGTAGAGATTAGGAAAATGAAACTAGTTTAAAACCAACCCAA
AAAGAGTAACTTATAAAATAACTGAAAATTTAAATAAAAGAATTATGAAAATACAAATTA
AATGAATATTTTTTGGTTCTTCCCTTGATAAAATTTTGACCTTAAATAATAAGAATCAAA
TTCGTAATTAGTTAATTGCAGATAACATGAAACTATACTATAAAAAAATATATATATATA
TATATAACTATACTAGTTTAGATGATAAAATATGTAAGTCTATTCGCACTTACTTAAAAA
CAAAATATACCATTTAATTTGTTGAATTAGAGAATCATGTACCTTGTACTTTGGGCCATA
TTTTCCCGCTCTATATACATATAAAACAAATAAATATAGAATTCGAATAAAAAACATATA
TAAATTAAAGAAAAATATAGAATCGGGATATATTCAATTGTAAATGGCTTCATG
   
Sequence Name 26-K018967-022-007-C10C-8409
GenBank Accession LN497710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K018967-022-007-C10C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATTCTATATTTTTCT
TTAATTTATCTATGTTTTTTATTCGAATTCTATATTTATTTGTTTTATATGTATATAGAG
CTGGAAAATATGGCCCAAAGTACAAGGTACATGATTCTCTAATTCAACAAATTAAATGGT
ATATTTTGTTTTTAAGTAAGTGCGAATAGACTTACATATTTTATCATCTAAACTAGTATA
GTTATATATATATATATATATTTTTTTATAGTATAGTTTCATGTTATCTGCAATTAACTA
ATTACGAATTTGATTCTTATTATTTAAGGGCAAAATTTTATCAAGGGAAGAACCAAAAAA
TATTCATTTAATTTGTATTTTCATAATTCTTTTATTTAAATTTTCAGTTATTTTATAAGT
TACTCTTTTTGGGTTGGTTTTAAACTAGTTTCATTTTCCTAATCTCTACAGTTTAATTGA
CATCTAGCTTTTATCAGTTAATCTTAGAAGTACCGTTTATTAACACTTTTAACAAACAAA
CAAAAAATTGTAACGCTTTTAAGTTTTAACAACAAATAAATTATATAATTTATAGGGCTT
ATCAGAAAGAAAGAAAAAAATACTCTAGAAATTGAAAAAGATCCACACATTGTGCGTGTA
ATAATAGNACAAAACCAAAAAGCCTATCTCATGAC
Primers used for PCR FM57 - 3144, show primer details
   
Sequence Name 09-K029297-022-007-C10C-3144
GenBank Accession LN490046 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K029297-022-007-C10C-3144
CTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGG
TACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCGTTTCTCCGCCTTC
GTTTCCCGCATTCGAATACATCATACTCACTTGAATAAATTTGACACACTTATATACTTC
AAAAAAATAAAAATAAAAATATTGGGGAAAAATCTAGCCATGTCGACGCTGACGCATGAG
CATGGAGGAGAAATAGGCACGATTTTCAAATGTTATTTTGATAAAACCCACGAGGTTTAA
TGTGAGTGGCTAAGAAAATTTGACACGTGCACAAAAACTCTTAGCTACGTCTAAGAACAA
ACTTCGATCAAGCTTATCACAAAGGAAATAAACACTTTTTGCTTTAGTCACCAAATGTCT
T
   
Sequence Name 10-K029297-022-007-C10C-FM57
GenBank Accession LN490520 [GenBank]
Sequencing primer gene specific primer, FM57: 5'-CAAGACATTTGGTGACTAAAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K029297-022-007-C10C-FM57
GCTTGATCGAGTTTGTTCTAGACGTAGCTAAAGAGTTTTTGTGCACGTGTCAAATTTTCT
TAGCCACTCACATTAAACCTCGTGGGGTTTTATCAAAATAACATTTGAAAATCGTGCCTA
TTTCTCCTCCATGCTCATGCGTCAGCGTCGACATGGCTAGATTTTTCCCCAATATTTTTA
TTTTTATTTTTTTGAAGTATATAAGTGTGTCAAATTTATTCAAGTGAGTATGATGTATTC
GAATGCGGGAAACGAAGGCGGAGAAACGATAGTTTAAACCGAAGGCGGGAAACGACAATC
TGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGT
CTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAAT
CCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGT

Last Updated on 06.12.2018 16:11