Sequences from confirmation amplicon


Confirmation sequences for line 032D06 and hit At4g09460 are shown.
Line ID 032D06
Locus At4g09460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5993506
Primers used for PCR DR01 - 8474, show primer details
   
Sequence Name 57-K018752-022-032-D06C-DR01
GenBank Accession LN509273 [GenBank]
Sequencing primer gene specific primer, DR01: 5'-TGGTCCCCATTATAACCTAATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K018752-022-032-D06C-DR01
AGAAGCTTCTGCTTCAAAAAACATATAAAAACTATGATCTATACCAACATGTAAAAATGG
ATAACGGTTGATCCATAAGAAAGAGTCCAAAAGGGACCAACCATGTTCACATTTCACTCC
TACATTTATACTTCTACATTTTATAACAATATTGTCAAAATAAAATTCAAATTACTCATT
TGCATTCCTAGACAATGGTGTAATATTCTTATATATTTCCATAAAATTATTTTGGAAACC
ATGTATCGTTTTTTTTCTTTTTCTTCAAAAGGTAATATGAACAAAAACTAAAACAAAAAT
ACCAAAGAAACTGAAGTAAAGAATTTCTATATTTATCCAAAAGGACTCAAGCAAAAAAAA
AAATGAAGTAACAAAACCCATTTTTTGGGATTTTTTTGACAAATTATTACAACAGTGACA
AACGCGCCTCCATCACCGGGTCGCACCACGGTTTAAAATCAGCCGATGACCCGACTCCGA
CTATCCGACCCGACCAGGATCCAAACCCAAGAGTTAAATCCAAATTCAGCTTTATATGTC
CTGAATTA
   
Sequence Name 61-K018752-022-032-D06C-8409
GenBank Accession LN510397 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K018752-022-032-D06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAGATTGCTGATCCGGCAGAATG
GGGAGTGTTATTATAGTGATAATTCAGGACATATAAAGCTGAATTTGGATTTAACTCTTG
GGTTTGGATCCTGGTCGGGTCGGATAGTCGGAGTCGGGTCATCGGCTGATTCTAAACCGT
GGTGCGACCCGGTGATGGAGGCGCGTTTGTCACTGTTGTAATAATTTGTCAAAAAAATCC
CAAAAAATGGGTTTTGTTACTTCATTTTTTTTTTTGCTTGAGTCCTTTTGGATAAATATA
GAAATTCTTTACTTCAGTTTCTTTGGAATTTTTGTTTTAGTTTTTGTTCATATTACCTTT
TGAAGAAAAAGAAAAAAAACGATACATGGTTTCCAAA
Primers used for PCR 25wo - 3144, show primer details
   
Sequence Name 28-K118496-0022-032-D06C-25wo
GenBank Accession LN499051 [GenBank]
Sequencing primer gene specific primer, 25wo: 5'-CTACCTAATCTCCATTACCCCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118496-0022-032-D06C-25wo
TTTATCTCCCATAGTCACATGTACATATACCAAACTTAGTCAAAGTCTCCTCCTTTTTAT
ATAACCCACAAATTGATCTCTTCTCTCTTCTCTTACACCATCTTCTTCATTCACCATGGG
AAGATCTCCTTGTTGTGAAAAAGCTCACACAAACAAAGGAGCTTGGACTAAAGAAGAAGA
TCAACGTCTCGTAGATTATATCCGTAATCACGGTGAAGGTTGTTGGCGTTCTCTTCCTAA
ATCCGCTGGATTGTTGCGTTGTGGTAAAAGTTGTAGATTGAGATGGATTAATTACCTTCG
TCCTGATCTTAAACGTGGTAATTTTACTGATGATGAAGATCAAATCATCATCAAACTCCA
TAGCTTACTCGGTAACAAGTACGATAACTTTTTCCTCTCTTTCTCTTCTTCTAAAAAAAC
AGAGTGAAAATAGAAAAACAGAGTCACTTTTTTTTTTCTCCCTACTCTGGTTTCTAGGAA
AAAAATTTCAACTTTCTAGGAAACAGAGAGAAAATAGAAACTGGGTCACTTTGTTTTCCG
GCTCTTTCTTCTTCTTTGATCTTTCCCAGGAAAAAAGTTTCAACTTTCTAGGAAACAAAG
TCAACAAAAATGGCTTACCCTGACATAAAACGACAATCTGATAGTTTAAACCGAAGGCGG
GAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATA
TAGAGAAAGGGTCTTGCAAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGT
   
Sequence Name 04-K118495-0022-032-D06C-35St
GenBank Accession LN488117 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118495-0022-032-D06C-35St
TCCACTATCCTTGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGCGTACAG
GGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGATTGTCGTTT
TATGTCAGGGTAAGCCATTTTTGTCGACTCTGTTTCCTAGAAAGTTGAAACTTTTTTCCT
GGGAAAGATCAAAGAAGAAGAAAGAGCAGGAAAACAAAGTGACCCAGTTTCTATTTTCTC
TCTGTTTCCTAGAAAGTTGAAATCTTTTTCCTAGAAACAAGAGTAGGGAGAAAAAAAAAA
GTGACTCTGTTTTTCTATTTTCACTCTGTTTTTTTAGAAGAAGAGAAAGAGAGGAAAAAG
TTATCGTACTTGTTACCGAGTAAGCTATGGAGTTTGATGATGATTTGATCTTCATCATCA
GTAAAATTACCACGTTTAAAATCAGGACGAAGGTAATTAATCCATCTCAATCTACAACTT
TTACCACAACGCAACAATCCAGCGGATTTAGGAAAAGAACGCCAACAACCTTCACCGTGA
TTACGGATATAATCTACAAGACGTTGATCTTCTTCTTTAGTCCAAGCTCCTTTGTTTGTG
TGAGCTTTTTCACAACAAGGAGATCTTCCCATGGTGAATGAAGAAAATGGTGTAAGAGAA
GAGAGAAGAGATCAATTTGTGGGTTATATAAAAAGGAGGAGACTTTGACTAATTTTGGTA
TATGTACATGTGACTATGTGGAGATAAAGTGAGGCTGTGCTTTGCTGTGTTGTGTGTGGG

Last Updated on 06.12.2018 16:11