Sequences from confirmation amplicon


Confirmation sequences for line 037C03 and hit At3g47810 are shown.
Line ID 037C03
Locus At3g47810 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17637852
Primers used for PCR IH04 - 8474, show primer details
   
Sequence Name 05-K023622-022-037-C03C-8409
GenBank Accession LN488181 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>05-K023622-022-037-C03C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATATTCAAAGCCTGTTTAAAT
TATCATGCAAAATAACAAGCGAGCCAACGCTGAAACTCACTGGGTAAGGGACACACACCA
ACAAAGCAATGAAGTAAAGGCAAGCAAAATTTGGTTTTCTAGTCTGAGAATTCTGATAAA
CAGATGATGTATGATACGAGGGATTATTCGTATGCTGCAAATGCTAGCGTCTAACTCGTA
AAGATCATGATGAAATGCACAAAGAAGGGAAACTGAACCTGGTGGCCATGACACAATCCC
AGCTTGAATTGCCCAATAGTCAAGGTTTTATTCTCAGGGTATCGCGCATCTTCGTCAAAC
TCCCCTCGAACTATATGCAAATCAGGGCAGATAGTCTTCAAGTAGTCATGGATTTCCTAC
AA
   
Sequence Name 06-K023622-022-037-C03C-IH04
GenBank Accession LN488629 [GenBank]
Sequencing primer gene specific primer, IH04: 5'-TTTTTGACTGTTGTAGGAAATCCA-3'
Sequence (A. th genome BLAST matches underlined)
>06-K023622-022-037-C03C-IH04
AAGACTATCTGCCCTGATTTGCATATAGTTCGAGGGGAGTTTGACGAAGATGCGCGATAC
CCTGAGAATAAAACCTTGACTATTGGGCAATTCAAGCTGGGATTGTGTCATGGCCACCAG
GTTCAGTTTCCCTTCTTTGTGCATTTCATCATGATCTTTACGAGTTAGACGCTAGCATTT
GCAGCATACGAATAATCCCTCGTATCATACATCATCTGTTTATCAGAATTCTCAGACTAG
AAAACCAAATTTTGCTTGCCTTTACTTCATTGCTTTGTTGGTGTGTGTCCCTTACCCAGT
GAGTTTCAGCGTTGGCTCGCTTGTTATTTTGCATGATAATTTAAACAGGCTTTGAATATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA

Last Updated on 23.10.2014 13:25